茯苓95%乙醇提取物三萜类化学成分的UPLC-IT-TOF/MS分析  

Analysis of Triterpene Chemical Constituents in Poria cocos 95%Ethanol Extract via UPLC-IT-TOF/MS

作　　者：陈兴龙 张敏 杨琪瑶 罗永谋 曹婷婷 唐丽萍 李勇 CHEN Xing-long;ZHANG Min;YANG Qi-yao;LUO Yong-mou;CAO Ting-ting;TANG Li-ping;LI Yong(College of Traditional Chinese Medicine&Yunnan Key Laboratory of Southern Medicine Utilization,Yunnan University of Chinese Medicine,Kunming Yunnan 650504;Dept.of Ophthalmology,The First Hospital of Kunming,Kunming Yunnan 650224;School of Pharmaceutical Science and Yunnan Key Laboratory of Pharmacology of Natural Products,Kunming Medical University,Kunming Yunnan 650500,China)

机构地区：[1]云南中医药大学中药学院暨云南省南药可持续利用重点实验室,云南昆明650504 [2]昆明医科大学附属甘美医院/昆明市第一人民医院眼科,云南昆明650224 [3]昆明医科大学药学院暨云南省天然药物药理重点实验室,云南昆明650500

出　　处：《昆明医科大学学报》2021年第11期1-8,共8页Journal of Kunming Medical University

基　　金：国家自然科学基金资助项目(31860087);云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目(2019FE001(-271))。

摘　　要：目的采用超高效液相色谱串联离子阱飞行时间质谱(UPLC-IT-TOF/MS)技术定性分析茯苓95%乙醇提取物的三萜类化学成分。方法采用UHPLC XB-C18色谱柱,甲酸/水(0.05/100,v/v)-乙腈作为流动相,梯度洗脱,流速为0.20 mL/min;紫外检测波长范围为190~400 nm。使用ESI离子源,三氟乙酸钠校正,电喷雾正负离子同时检测,Shimadzu Composition Formula Predictor软件定性分析茯苓三萜类化合物。结果通过多级质谱数据分析,结合氮规则和Scifinder数据库检索,从茯苓醇提取物中鉴定了22个三萜类成分,结构类型包括羊毛甾-8-烯型三萜、羊毛甾-7,9(11)-二烯型三萜和其他羊毛甾三萜类型。结论通过LC-MS快速分析了茯苓醇提取物中三萜类成分,为其药效物质基础和质量控制提供了依据。Objective To evaluate the triterpene chemical constituents in Poria cocos 95%ethanol extract by ultra-performance liquid chromatography ion trap time-of-flight mass spectrometry(UPLC-IT-TOF/MS)method.Methods UPLC was performed with the UHPLC XB-C18 and methanoic acid/water(0.05/100,v/v)-acetonitrile solution(gradient elution)was employed with a flow rate of 0.2 mL·min-1.The detection wavelength range was 190~400 nm.Triterpene chemical compositions in Poria cocos were qualitatively analyzed using ESI ion with electrospray positive and negative ions simultaneous detection,sodium trifluoroacetace correction and Shimadzu Composition Formula Predictor software.Results Through the analysis of the multistage tandem mass spectrometry,combined with the application of nitrogen rule and the Scifinder database search,22 triterpenes with lanostan-8-en,lanostan-7,9(11)-diene type and other lanostane triterpene were identified.Conclusion This study quickly analyzed the triterpenes in the Poria cocos ethanol extract via LC-MS,which provided the basis for the pharmacodyamic material basis and quality control.

关 键 词：茯苓 三萜 UPLC-IT-TOF/MS 

分 类 号：R284.1[医药卫生—中药学]