低温诱导白灵侧耳原基形成的生理变化  

Physiological Changes of Primordium Formation of Pleurotus tuoliensis Under Low Temperature

作　　者：王晶 许子洁 常晓宁 胡宝 王春霞 WANG Jing;XU Zijie;CHANG Xiaoning;HU Bao;WANG Chunxia(College of Landscape and Ecological Engineering,Hebei University of Engineering,Handan,Hebei 056038)

机构地区：[1]河北工程大学园林与生态工程学院,河北邯郸056038

出　　处：《北方园艺》2021年第21期123-129,共7页Northern Horticulture

基　　金：河北省现代农业产业技术体系食用菌创新团队建设专项资金资助项目(HBCT2018050202);河北省重点研发计划项目现代种业科技专项资助项目(19222903D);邯郸市2020年度哲学社会科学规划资助项目(2020027)。

摘　　要：以白灵侧耳(Pleurotus tuoliensis)菌株"中农1号"为试材,采用蒽酮比色等方法,测定了4个处理(CK、T1、T2、T3)中保护酶活性、丙二醛含量、可溶性糖含量等生理指标,研究了低温诱导白灵侧耳原基形成过程中的生理变化情况,以期为白灵侧耳的实际栽培与生产提供参考依据。结果表明:各处理的丙二醛(MDA)含量呈先降低后升高的趋势;3种保护酶(SOD、POD、CAT)活性均先升高后降低,其中T1处理中SOD和POD活性最高;T1与T2处理中CAT活性无显著差异,但均显著高于CK(P<0.05);可溶性糖含量呈先升高后降低的趋势,可溶性糖含量均显著高于CK(P<0.05);各处理中游离脯氨酸含量差异显著(P<0.05),其中T1处理中含量最高。综合分析表明,在低温诱导过程中保护酶活性、丙二醛含量、可溶性糖含量等生理指标均有变化,其中保护酶、可溶性蛋白质及游离脯氨酸含量变化较大。Taking the strain‘Zhongnong No.1’of Pleurotus tuoliensis as the test material,the physiological indexes such as the protective enzymes activities,MDA content and soluble sugar content in four treatments(CK,T1,T2,T3)were determined.The physiological changes were studied,in order to provide reference basis and technical support for the cultivation and production of Pleurotus tuoliensis.The results showed that MDA content decreased at first and then increased.The activities of SOD,POD and CAT increased firstly and then decreased.The activities of SOD and POD were the highest in T1 treatment.There was no significant difference in CAT activity between T1 and T2 treatments,but both of them were significantly higher than that in CK(P<0.05).The content of soluble sugar increased at first and then decreased,and the content of soluble sugar in all treatments was significantly higher than that in CK(P<0.05).The content of free proline in all treatments was significantly different(P<0.05),and the content of free proline in T1 treatment was the highest.Comprehensive analysis showed that the physiological indexes were changed during the induction of low temperature,and the activities of protective enzymes,the contents of soluble protein and free proline were changed greatly.

关 键 词：白灵侧耳 低温诱导 原基形成 生理指标 

分 类 号：S646[农业科学—蔬菜学]