柳树AP2/ERF基因家族全基因组鉴定和表达分析  

Genome-wide identification and characterization of the AP 2/ERF gene family in willow

作　　者：戴晓港 李淑娴 Dai Xiaogang;Li Shuxian(The Key Laboratory of Tree Genetic Improvement and Biotechnology of Jiangsu Province,The Southern Poplar Germplasm Engineering and Wood Processing Technology Center, College of Forestry, Nanjing Forestry University, Nanjing 210037, China)

机构地区：[1]南京林业大学林学院,南方杨树工程技术研究中心,江苏省杨树种质创新与品种改良重点实验室,江苏南京210037

出　　处：《江苏林业科技》2021年第5期1-12,52,共13页Journal of Jiangsu Forestry Science & Technology

基　　金：国家自然科学基金项目“簸箕柳组培再生体系及农杆菌介导的遗传转化研究”(31500533)。

摘　　要：APETALA2/ethylene-responsive factor(AP2/ERF)转录因子在调控植物的生长和发育、应生物胁迫和非生物胁迫等方面扮演着至关重要的角色。该文对簸箕柳基因组中AP2/ERF基因进行查找,分析了该基因家族的保守结构域、基因结构、启动子区域顺式作用元件、基因在染色体上的分布及其在干旱胁迫下的差异表达。分析结果发现,簸箕柳中共含有208个AP2/ERF基因。根据系统发育树聚类分析的结果,这些基因可分为AP2,RAV,ERF和Soloist 4个亚类;基因结构分析表明,AP2和Soloist基因内含子较多,而多数ERF和所有RAV基因都无内含子;对启动子区域顺式作用元件分析,发现簸箕柳AP2/ERF家族基因启动子区域富含响应非生物胁迫的相关元件;对基因在染色体上分布分析表明,簸箕柳AP2/ERF基因在不同染色体上分布不均匀,其中42个基因的扩张与串联复制有关。利用蒿柳杂交子代的干旱胁迫和对照样品的转录组序列进行了差异表达分析,发现了5个AP2/ERF基因在干旱胁迫下显著上调表达。分析结果为深入研究柳树AP2/ERF基因家族的功能提供了重要参考。APETALA2/ethylene-responsive factor(AP2/ERF)transcription factors perform a crucial role in various biological processes,such as regulating plant growth and development,responding to abiotic and biotic stresses.In this study,we identified AP2/ERF superfamily genes in Salix suchowensis,and analyzed their conserved motif,gene structure,cis-regulatory elements in the promoter,chromosome distribution and expression patterns under drought stress.A total of 208 AP2/ERF genes were identified in S.suchowensis genome.The identified AP2/ERF genes were classified into 4 major clades including AP2,ERF,RAV and Soloist based on the phylogenetic trees.Gene structural analysis showed that the genes in AP2 and Soloist clades had more introns than those in ERF and RAV clades.Cis-regulatory elements which are related to abiotic stresses were identified and enriched in the promotor of the SsAP2/ERF genes.Plotting their distribution showed that the SsAP2/ERFs unevenly scattered on the 19 chromosomes of S.suchowensis,with 42(20.2%)SsAP2/ERF genes related to tandem duplication.The transcriptome reads derived from S.viminalis progeny with drought treatment were mapped on to the genome of S.suchowensis and five AP2/ERF genes were identified which were up-regulated in drought stress compared with control treatment.The above results will provide a valuable clue for further exploring the functional of willow AP2/ERF genes.

关 键 词：簸箕柳 AP2/ERF基因家族 分子进化 干旱胁迫 基因表达 

分 类 号：Q756[生物学—分子生物学] S792.12[农业科学—林木遗传育种]