基于不同氮源培养条件的巴氏芽孢杆菌脲酶功能转录组分析  

Transcriptome Analyses Reveal The Urease Function of Sporosarcina pasteurii Based on Different Nitrogen Source Culture Conditions

作　　者：裴迪 刘志明 胡碧茹[1] 吴文健[1] PEI Di;LIU Zhi-Ming;HU Bi-Ru;WU Wen-Jian(College of Science,National University of Defense Technology,Changsha 410073,China;Institute of Chemical Defense,Academy of Military Science,Beijing 102205,China)

机构地区：[1]国防科技大学文理学院,长沙410073 [2]军事科学院防化研究院,北京102205

出　　处：《生物化学与生物物理进展》2021年第9期1063-1076,共14页Progress In Biochemistry and Biophysics

摘　　要：巴氏芽孢杆菌是源于土壤的革兰氏阳性菌,人们利用其高效的脲酶活性诱导产生碳酸钙的现象开发了多种应用场景.然而,巴氏芽孢杆菌的生物矿化相关代谢机制还不够明确,尤其是对在矿化作用中发挥核心作用的脲酶基因结构、表达调控机制及关联代谢等方面的研究相对较少.当前,巴氏芽孢杆菌应用研究中面临的矿化反应不可控性及不稳定性等问题都源于脲酶代谢机制的研究匮乏.因此,进一步揭示巴氏芽孢杆菌脲酶的基因信息、表达调控机制及相关代谢机理迫在眉睫.本文通过转录组测序,对比了4种培养条件下巴氏芽孢杆菌的生长情况和基因表达情况,解析了脲酶的代谢机制,结果进一步证明ATP合成与脲酶表达及尿素水解相关联,最终预测了脲酶的双操纵子结构.Sporosarcina pasteurii(S. pasteurii) is a kind of Gram-positive bacteria from soil. Various applications have been developed based on the efficient urease activity that can induce the precipitation of calcium carbonate. However, the metabolic mechanism related to biomineralization of S. pasteurii has not been clearly elucidated. Especially, there are few studies on the gene structure of urease, regulation mechanism of expression and associated metabolism, which play key roles in biomineralization. Nowadays, the uncontrollability and instability of biomineralization reactions in the applications of S. pasteurii root in the lack of research on urease metabolisms. Therefore, it is urgent to further reveal the gene information, expression regulation and related metabolism of urease in S. pasteurii. In this paper, we compared the growth and gene expression of S.pasteurii BNCC 337394 under four different culture conditions through high-throughput transcriptome analyses.The four medium conditions were:(1) control group with yeast extract of 20 g/L;(2) ammonium group with yeast extract of 20 g/L and ammonium chloride of 10 g/L;(3) urea group with yeast extract of 20 g/L and urea of 5.62 g/L;(4) ammonium Tris group with yeast extract of 20 g/L, ammonium chloride of 10 g/L and Tris of 15.75 g/L.Transcriptome data were analyzed by bioinformatics methods of differential gene expression analysis, GO enrichment analysis, KEGG enrichment analysis and operon prediction. The results showed that there were significant differences in the growth of S. pasteurii among the four conditions. The bacteria could not grow and reproduce normally in the control group. There was a growth delay in the ammonium Tris group. The growth rate and trend of the ammonium group and the urea group with the same nitrogen content were similar. A total of 3 090 genes were generated and expressed in S. pasteurii in the experiment. There were 1 152 differentially expressed genes(DEGs) between the ammonium group and the control group, of which 411 were up-reg

关 键 词：巴氏芽孢杆菌 转录组 操纵子 脲酶 ATP 

分 类 号：Q819[生物学—生物工程]