玫瑰花青素对小鼠抗疲劳及抗氧化研究  

Study on Anti-fatigue and Anti-oxidation Effects of Rose Anthocyanin in Mice

作　　者：程美玲 黄鑫 安曈昕[2] 范江平[3] 李建宾[2] 希从芳[2] CHENG Meiling;HUANG Xin;AN Tongxin;FAN Jiangping;LI Jianbin;XI Congfang(College of Veterinary Medicine,Yunnan Agricultural University,Kunming 650201,China;College of Agronomy and Biotechnology,Yunnan Agricultural University,Kunming 650201,China;College of Food Science and Technology,Yunnan Agricultural University,Kunming 650201,China)

机构地区：[1]云南农业大学动物医学院,云南昆明650201 [2]云南农业大学农学与生物技术学院,云南昆明650201 [3]云南农业大学食品科学技术学院,云南昆明650201

出　　处：《云南农业大学学报（自然科学版）》2021年第6期956-961,共6页Journal of Yunnan Agricultural University:Natural Science

基　　金：云南省重点研发计划项目(2016BC014)。

摘　　要：【目的】研究玫瑰花青素抗疲劳和抗氧化功效。【方法】通过灌胃给予健康雄性昆明小鼠低、中、高剂量(依次为100、150和200 mg/kg)玫瑰花青素,30 d后负重游泳;测定小鼠负重游泳时间、血清和肝脏中尿素氮(BUN)、血乳酸(BLA)和丙二醛(MDA)含量、乳酸脱氢酶(LDH)、谷胱甘肽过氧化物酶(GSH-Px)和总超氧化物歧化酶(T-SOD)活性以及总抗氧化能力(T-AOC)等生化指标;根据线性方程分析玫瑰花青素总抗氧化能力与其抗疲劳功效的关系。【结果】与对照组相比,低、中、高剂量玫瑰花青素使小鼠负重游泳时间极显著增加(P<0.01);低、中、高剂量组小鼠血液中BLA和BUN含量显著降低(P<0.05或P<0.01),LDH活性极显著增强(P<0.01);低、中、高剂量组小鼠血清和肝脏中的MDA含量均降低,GSH-Px和T-SOD活性均显著增加(P<0.05或P<0.01),T-AOC显著提高(P<0.05或P<0.01)。相关性分析表明:随着小鼠血液中TAOC增加,BLA和BUN含量降低,LDH活性升高。【结论】玫瑰花青素能有效增加小鼠体内抗氧化能力,缓解疲劳;抗疲劳和抗氧化功效与玫瑰花青素提取物灌胃剂量存在依赖关系。[Purpose]To study the anti-oxidation and anti-fatigue effects of rose anthocyanins.[Methods]Male healthy Kunming mice were given low(100 mg/kg),medium(150 mg/kg)and high(200 mg/kg)dose rose anthocyanins for 30 days.After weight-bearing swimming,the time of weight-bearing swimming was measured,and the biochemical indexes in serum and liver such as the contents of blood urea nitrogen(BUN),blood lactic acid(BLA)and malondialdehyde(MDA),the activities of lactic dehydrogenase(LDH),glutathione peroxide dismutase(GSH-Px)and total superoxide dismutase(T-SOD),and total anti-oxidant capacity(T-AOC)were measured.According to the linear equation,the relationship between anti-oxidant capacity and anti-fatigue effect of rose anthocyanins was analyzed.[Results]Compared with the control group,low,medium and high dose of rose anthocyanins extremely significantly prolonged the swimming time of mice with load(P<0.01);the contents of BLA and BUN in the blood of mice with low,medium and high dose of rose anthocyanins significantly decreased(P<0.05 or P<0.01),but the activity of LDH extremely significantly increased(P<0.01);the contents of MDA in the serum and liver of mice with low,medium and high dose of rose anthocyanins decreased,the activities of GSH-Px and T-SOD increased significantly(P<0.05,P<0.01),the T-AOC significantly increased(P<0.05,P<0.01).The correlation analysis showed that with the increase of T-AOC in mice,the contents of BLA and BUN decreased,and the activity of LDH increased.[Conclusion]Rose anthocyanins can effectively increase the anti-oxidation ability and relieve fatigue,and the anti-oxidation and anti-fatigue effects are dependent on the dose of gavage.

关 键 词：玫瑰花青素 抗疲劳 抗氧化 小鼠 

分 类 号：S685.120.99[农业科学—观赏园艺]