地衣芽孢杆菌对热应激公兔肾上腺结构及血浆皮质醇含量的影响  

Effect of Bacillus licheniformis on the Heat Stress to Adrenal Gland Structure and Plasma CORT Content in Male Rabbits

作　　者：韩昱 张立永[2] 张富梅[2] HAN Yu;ZHANG Liyong;ZHANG Fumei(Laboratory Animal Center,Hebei North University,Zhangjiakou 075000,China;College of Animal Science and Technology,Hebei North University,Zhangjiakou 075000,China)

机构地区：[1]河北北方学院实验动物中心,河北张家口075000 [2]河北北方学院动物科技学院,河北张家口075000

出　　处：《云南农业大学学报（自然科学版）》2021年第6期962-968,共7页Journal of Yunnan Agricultural University:Natural Science

基　　金：河北省科技计划项目(12226613,15226604D)。

摘　　要：【目的】研究地衣芽孢杆菌(Bacillus licheniformis)缓解种公兔热应激的相关机制。【方法】选用年龄相近、体况良好的种公兔90只,随机均分为常温对照组(N)、热应激组(H)和热应激+地衣芽孢杆菌组(H+B)。N组和H组饲喂基础饲粮,H+B组饲喂基础饲粮+地衣芽孢杆菌1000 mg/kg,H组和H+B组均模拟炎热夏季温度进行热应激刺激,试验30 d后测定公兔肾上腺指数和血浆皮质醇(CORT)含量,试验结束后制作肾上腺组织切片,用光镜和电镜观察肾上腺结构变化。【结果】H组和H+B组的肾上腺质量和肾上腺指数极显著高于N组(P<0.01),H+B组也极显著低于H组(P<0.01)。肾上腺结构观察显示:H组肾上腺被膜厚度减小,细胞团直径减小,超微结构显示肾上腺皮质和髓质结构严重受损,而H+B组肾上腺结构得到明显改善。血浆CORT含量显示:试验初期(12 h和3 d),H组的CORT水平极显著升高(P<0.01),但10 d后开始下降;H+B组的CORT水平虽在初期也表现升高,但升高缓慢,至中后期即趋于正常水平,试验30 d时与N组无显著差异(P>0.05)。【结论】饲粮中添加地衣芽孢杆菌可以缓解热应激刺激对种公兔肾上腺造成的损伤,有利于维持其正常生殖功能。[Purpose]To study the mechanism of Bacillus licheniformis for alleviating heat stress in male rabbits.[Method]Ninety male rabbits of similar age and good body condition were selected and randomly divided into normal temperature control group(N),heat stress group(H)and heat stress+B.licheniformis group(H+B).Group N and group H were fed with basic diets.Group H+B was fed with a basic diet+B.licheniformis 1000 mg/kg.Group H and group H+B were given heat stress stimulation to simulate hot summer temperature.The test period was 30 days.The adrenal index and content of CORT in the plasma of each group were measured.After the test,sections were made and the changes in adrenal gland structure were observed by light and electron microscopes.[Results]Adrenal weight and adrenal index in group H and group H+B were extremely significantly higher than those in group N(P<0.01).Group H+B was extremely significantly lower than the group H(P<0.01).Observation of adrenal gland structure showed that in group H,the thickness of the adrenal capsule was reduced,the diameter of the cell mass was reduced,and the structure of the adrenal cortex and medulla was severely damaged.Adrenal gland structure in group H+B was obviously improved.The plasma CORT content showed that at the beginning of the experiment(12 hours and 3 days),the CORT level in group H increased significantly(P<0.01),but began to decrease after 10 days.Although the CORT level of group H+B also increased at the beginning,it increased slowly and reached the normal level in the middle and late stages,and there was no significant difference from group N at the 30th day of the test(P>0.05).[Conclusion]The addition of B.licheniformis to the diet can alleviate the damage caused by heat stress stimuli to male rabbits and help maintain their normal reproductive function.

关 键 词：地衣芽孢杆菌 热应激 公兔 肾上腺 血浆皮质醇 

分 类 号：S816.73[农业科学—饲料科学]