云烟87内生真菌多样性及其促生作用评估  

Diversity of Endophytic Fungi from Yunyan87 and Evaluation of Growth-promoting

作　　者：谢佳伟 彭小祠 陈小巧 谭明华 刘彦中[1] 郝冰 XIE Jiawei;PENG Xiaoci;CHEN Xiaoqiao;TAN Minghua;LIU Yanzhong;HAO Bing(College of Tobacco Science,Yunnan Agricultural University,Kunming 650201,China)

机构地区：[1]云南农业大学烟草学院,云南昆明650201

出　　处：《云南农业大学学报（自然科学版）》2021年第6期1000-1008,共9页Journal of Yunnan Agricultural University:Natural Science

基　　金：云南中烟工业有限责任公司科技项目(滇烟工科[2013]494号)。

摘　　要：【目的】对云南省主栽烟草品种云烟87内生真菌进行分离和鉴定,阐明云烟87内生真菌的群落结构和多样性,并评估其对不同品种烟苗的促生作用。【方法】采用表面消毒和组织分离法分离云烟87成熟烟株内生真菌,测定浇灌发酵液后云烟87、云烟97、K326和红花大金元烟苗的鲜质量、根鲜质量、地上部鲜质量、株高、最大叶长、最大叶宽和根长等指标以评估其促生作用。【结果】共分离得到29属53种内生真菌。筛选出具有显著促进烟草生长的菌株3株,分别为尖孢镰刀菌(Fusarium oxysporum)、土曲霉(Aspergillus terreus)和朱黄篮状菌(Talaromyces minioluteus),3种内生真菌在云烟87中的菌群密集度较高。尖孢镰刀菌可提高云烟97烟苗的株高、鲜质量及地上部鲜质量,分别比对照增加107.64%、153.97%和167.09%;土曲霉对云烟87烟苗生物量的影响最为显著,鲜质量、根鲜质量和地上鲜质量分别比对照增长172.85%、209.57%和169.18%,还可使红花大金元根鲜质量增长121.47%;朱黄篮状菌可提高云烟87和K326烟苗的株高,比对照分别增长71.66%和50.55%。【结论】云烟87具有丰富的内生真菌,且具有组织差异性。尖孢镰刀菌(001E-5)和土曲霉(001H-10)对云烟97、云烟87和K326的鲜质量提高均在91%以上。本研究为烟草促生菌剂的研发提供了一定的理论依据。[Purpose]The endophytic fungi community and diversity of Yunyan87,main tobacco cultivar in Yunnan Province and the growth promoting effects on different tobacco seedings were elucidated after isolation and identification.[Method]The endophytic fungi of mature tobacco plants were separated after surface disinfection and tissue separation.The effects of the growth promotion of endophytic fungi were evaluated by the fresh weight,root fresh weight,fresh weight of the aboveground,plant height,maximum leaf length,maximum leaf width and root length of the seedlings of Yunyan87,Yunyan97,K326 and Honghuadajinyuan after addition of the fermentation broth.[Results]A total of 53 endophytic fungi belonging to 29 genera were isolated and identified.Fusarium oxysporum,Aspergillus terreus and Talaromyces minioluteus showed significant promoting effect of tobacco growth were screened out,and the concentration of these three endophytic fungi in Yunyan87 were relatively high.Compared with the control group,F.oxysporum could increase the plant height,fresh weight and aboveground fresh weight of Yunyan97 seedling by 107.64%,153.97% and 167.09%,respectively.While A.terreus showed the most significant effect on increasing the biomass of Yunyan87 tobacco seedlings,the fresh weight,root fresh weight and aboveground fresh weight increased by 172.85%,209.57% and 169.18%,respectively.T.minioluteus increased the height of Yunyan87 and K326 tobacco seedlings by 71.66% and 50.55%.[Conclusion]There were abundant endophytic fungi living in Yunyan87 which had organizational differences.F.oxysporum(001E-5)and A.terreus(001H-10)increased the fresh weight of Yunyan97,Yunyan87 and K326 by over 91%.This study provided a theoretical basis for the development of tobacco growth promoting agents.

关 键 词：云烟87 内生真菌 分离鉴定 烟苗 促生作用 

分 类 号：S572.01[农业科学—烟草工业]