樟叶越桔幼茎愈伤组织诱导和不定根的再生  

Callus Induction and Adventitious Root Regeneration in Young Stem of Vaccinium dunalianum Wight

作　　者：李雪[1] 李国泽 杨玲[2] 唐军荣[2] 赵平[2] 丁勇[1] LI Xue;LI Guoze;YANG Ling;TANG Junrong;ZHAO Ping;DING Yong(Key Laboratory of Forest Biotechnology in Yunnan,Southwest Forestry University,Kunming 650224,China;Key Laboratory of State Forestry and Grassland Administration on Highly-efficient Utilization of Forestry Biomass Resources in Southwest China,Southwest Forestry University,Kunming 650224,China)

机构地区：[1]西南林业大学,云南省高校林木生物技术重点实验室,云南昆明650224 [2]西南林业大学,西南地区林业生物质资源高效利用国家林业与草原局重点实验室,云南昆明650224

出　　处：《云南农业大学学报（自然科学版）》2021年第6期1009-1016,共8页Journal of Yunnan Agricultural University:Natural Science

基　　金：国家自然科学基金项目(31960073,31460076);云南省高校林木生物技术重点实验室开放基金(51700201)。

摘　　要：【目的】建立樟叶越桔遗传转化体系,并为其重要次生代谢产物熊果苷及其衍生物的生产提供材料参考。【方法】以樟叶越桔组培苗幼嫩茎段为外植体,采用木本植物培养基(WPM)培养,研究不同质量浓度的2,4-二氯苯氧乙酸(2,4-D)和噻苯隆(TDZ)搭配对樟叶越桔茎段愈伤组织的诱导,并分析不同细胞分裂素及其质量浓度对愈伤组织再分化不定根的影响,获得樟叶越桔茎段愈伤组织诱导和愈伤组织再分化不定根的理想配方。【结果】樟叶越桔茎段愈伤组织诱导最佳培养基配方为WPM+2,4-D 2.0 mg/L+TDZ 0.1 mg/L+蔗糖30 g/L+琼脂5 g/L,诱导出的愈伤组织呈乳白色且质地紧实,诱导率可达100%。樟叶越桔愈伤组织再分化不定根的最佳配方为WPM+TDZ 0.5 mg/L+蔗糖30 g/L+琼脂5 g/L,不定根生根率高达96.7%,数量多、较长、整齐且均匀。【结论】成功建立了樟叶越桔幼嫩茎段愈伤组织诱导及愈伤组织再分化不定根的体系,对以愈伤组织和不定根为材料的樟叶越桔重要次生代谢产物的生产具有一定的参考意义。[Purpose]To establish the genetic transformation system of Vaccinium dunalianum Wight,and to provide material reference for the production of important secondary metabolites such as arbutin and its derivatives in V.dunalianum.[Method]The young stem segments of the tissue culture seedlings of V.dunalianum was taken as the explants,and the woody plant medium(WPM)was used in this study,the effects of different mass concentrations of 2,4-dichlorophenooxyacetic acid(2,4-D)and thidiazuron(TDZ)on callus induction and different cytokinins and mass concentrations on the differentiation of adventitious roots from callus were investigated in order to obtain the ideal formula of them.[Result]The optimal medium formula for callus induction in the stem section of V.dunalianum was WPM+2,4-D 2.0 mg/L+TDZ 0.1 mg/L+sucrose 30 g/L+agar 5 g/L,the induced callus was milky white and firm,and the induction rate was up to 100%.In the stage of callus re-differentiation,WPM+TDZ 0.5 mg/L+sucrose 30 g/L+agar 5 g/L was the best formula for the adventitious roots induction,the induction rate of adventitious roots was up to 96.7%,and adventitious roots were numerous,long,orderly and homogeneous.[Conclusion]This study successfully established a system of callus induction from the young stems of V.dunalianum and callus differentiated adventitious roots,to provided a reference for the research on the production of important secondary metabolites and plant nutrition of V.dunalianum in the later reserch using callus and adventitious roots.

关 键 词：樟叶越桔 愈伤组织 不定根 诱导 

分 类 号：Q813.1[生物学—生物工程]