不同丛枝菌根真菌促进资阳香橙幼苗生长研究  

Research on Arbuscular Mycorrhizal Fungi on the Growth of Ziyang Xiangcheng Seedling

作　　者：李果果[1] 刘金华 宋娟[3] 李铭燕 李冬萍[3] 刘升球 张金莲[3,6] 陈廷速 LI Guoguo;LIU Jinhua;SONG Juan;LI Mingyan;LI Dongping;LIU Shengqiu;ZHANG Jinlian;CHEN Tingsu(Horticulture Research Institute,Guangxi Academy of Agriculture Sciences,Nanning 530007,China;Zhejiang Yangshengtang Institute of Natural Medication Co.,Ltd.,Hangzhou 310007,China;Microbiology Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007,China;Guangxi Xiaozao Agriculture and Techonology Co.,Ltd.,Fangchenggang 538000,China;Guangxi Academy of Specialty Crops,Guilin 541004,China;School of Public Health,Xiamen University,Xiamen 361102,China)

机构地区：[1]广西农业科学院园艺研究所,广西南宁530007 [2]浙江养生堂天然药物研究所有限公司,浙江杭州310007 [3]广西农业科学院微生物研究所,广西南宁530007 [4]广西小藻农业科技有限公司,广西防城港538000 [5]广西特色作物研究院,广西桂林541004 [6]厦门大学公共卫生学院,福建厦门361102

出　　处：《云南农业大学学报（自然科学版）》2021年第6期1022-1027,共6页Journal of Yunnan Agricultural University:Natural Science

基　　金：广西科技基地和人才专项(桂科AD20159001);厦门大学联合项目(2017-21);广西科技重大专项(桂科AA17204046-4);广西自然科学基金(2017GXNSFBA198055);玉林市市校合作项目(YLSXZD2019016)。

摘　　要：【目的】筛选促进柑橘砧木资阳香橙盆栽幼苗生长的丛枝菌根真菌(arbuscular mycorrhizal fungi,AMF),为柑橘菌根种苗应用提供参考,也为利用菌根柑橘种苗抑制柑橘黄龙病探索新途径。【方法】采用盆栽试验,利用资阳香橙幼苗接种从广西土壤分离纯化获得的15株AMF菌株,研究不同AMF对根系的侵染及对植株的促生效应。【结果】15株AMF均能与资阳香橙根系形成共生关系。其中,黄雷德克囊霉1(Redeckera fulvum 1)对植株根系的侵染率最高,达到81.27%;脆无梗囊霉(Acaulospora delicata)的侵染率最低,为8.57%;15株菌株的平均侵染率为50.97%。幼套近明球囊霉(Claroideoglomus etunicatum)和摩西斗管囊霉(Funneliformis mosseae)可促进资阳香橙的生长,其株高、茎粗、叶片数和叶绿素SPAD值均显著高于对照(P<0.05)。【结论】幼套近明球囊霉和摩西斗管囊霉是促进资阳香橙生长的优势菌株。[Purpose]Arbuscular mycorrhizal fungi(AMF)were screened to promote the growth of citrus rootstock Ziyang Xiangcheng potted seedlings,which provided a reference for the application of mycorrhizal citrus seedlings,and also explored a new way for the use of mycorrhizal citrus seedlings to inhibit citrus Huanglongbing.[Method]To study the infection of different AMF on roots and their growth promoting effects on plants,Ziyang Xiangcheng seedlings were inoculated with 15 AMF,which were isolated and purified from soils in Guangxi.[Result]Fifteen AMF could form symbiotic relationship with the roots of Ziyang Xiangcheng.Among them,the highest infection rate was Redeckera fulvum 1 of 81.27%,the lowest infection rate was Acaulospora delicate of 8.57%,and the average infection rate of 15 AMF was 50.97%.Claroideoglomus etunicatum and Funneliformis mosseae could promoted the growth of Ziyang Xiangcheng,which was significantly higher than the control in plant height,stem diameter,leaf number and chlorophyll SPAD value(P<0.05).[Conclusion]C.etunicatum and F.mosseae were the superiority strains of Ziyang Xiangcheng.

关 键 词：丛枝菌根真菌 柑橘 资阳香橙 菌根侵染率 促进生长 

分 类 号：S666.401[农业科学—果树学]