不同质量浓度六氯苯胁迫下狭叶香蒲根系生物膜细菌群落结构研究  

Study of Bacterial Community Structure in Biofilm of Typha angustifolia Roots under Different Mass Concentrations Hexaehlorobenzen Stress

作　　者：陈海娇 付如鹏 何秀梅 张晋瑞 钟媛 余娇娇 CHEN Haijiao;FU Rupeng;HE Xiumei;ZHANG Jinrui;ZHONG Yuan;YU Jiaojiao(School of Chemical Biology and Environment,Yuxi Normal Universtiy,Yuxi 653100,China)

机构地区：[1]玉溪师范学院化学生物与环境学院,云南玉溪653100

出　　处：《云南农业大学学报（自然科学版）》2021年第6期1083-1091,共9页Journal of Yunnan Agricultural University:Natural Science

基　　金：云南省大学生创新创业训练计划项目(2019A11);云南省地方本科高校联合面上项目(2018FH001-041,2017FH001-043);云南省地方本科高校联合重点项目(2017FH001-125)。

摘　　要：【目的】研究不同质量浓度六氯苯(hexaehlorobenzen,HCB)胁迫下狭叶香蒲(Typha angustifolia)根系生物膜菌群结构。【方法】利用扫描电镜观察不同质量浓度(38和760μg/L)HCB富营养化水体中香蒲根系生物膜的形态结构;采用高通量测序技术对细菌16S rDNA基因进行序列测定。【结果】电镜结果显示:根系生物膜附着微生物主要呈椭圆形或杆状。经不同质量浓度HCB处理后,狭叶香蒲根系附着微生物的数量明显增加,随着水体HCB质量浓度升高其根系生物膜中的细菌数量降低。高通量测序结果表明:根系生物膜的优势菌门是变形菌门(Proteobacteria)、放线菌门(Actinobacteria)、绿弯菌门(Chloroflexi)、蓝藻菌门(Cyanobacteria)和厚壁菌门(Firmicutes)等。属水平优势菌群主要包括3类:(1)高效净化水质的光合菌群,如红微菌属(Rhodomicrobium)、红假单胞菌属(Rhodopseudomonas)、红长命菌属(Rubrivivax)、红芽生菌属(Rhodoblastus)和红细菌属(Rhodobacter)等;(2)以丛毛单胞菌属(Comamonas)、贪噬菌属(Variovorax)、不动杆菌属(Acinetobacter)、嗜酸菌属(Acidovorax)等为主的有机物降解微生物;(3)参与脱氮和氮循环的反硝化菌群,如贪噬菌属(Variovorax)、丛毛单胞菌属(Comamonas)、脱硫弧菌属(Desulfovibrio)、红细菌属(Rhodobacter)、不动杆菌属(Acinetobacter)、中慢生根瘤菌属(Mesorhizobium)和红长命菌属(Rubrivivax)等。PICRUSt功能预测结果表明:根系生物膜中菌群的主要代谢功能是碳水化合物、氨基酸以及能量代谢。【结论】38μg/L HCB的富营养化水体中狭叶香蒲根系细菌群落的丰富度和多样性均高于760μg/L HCB的富营养化水体根系细菌群落。此外,属水平优势菌群可能与富营养化水体的水质净化、有机物降解以及脱氮和氮循环有关。研究结果为富营养化水体有机污染物的去除机制提供了理论基础。[Purpose]To study the bacterial community structure in the biofilm of Typha angustifolia under different mass concentrations hexaehlorobenzen(HCB)stress.[Method]Biofilm morphology on root of T.angustifolia was monitored by using SEM and 16S rDNA gene of the bacteria by high-throughput sequencing used root samples under different mass concentrations(38 and 760μg/L)HCB stress.[Result]The results of SEM showed that the microorganisms attached to the root biofilm were elliptic or rod-shaped.After different mass concentrations of HCB treatment,the amount of microorganisms attached to T.angustifolia roots increased obviously,with the increase of HCB mass concentration in water,a small quantity of bacteria inhabited the biofilm.The results of high-throughput sequencing revealed that the dominant phyla of bacteria were Proteobacteria,Actinobacteria,Chloroflexi,Cyanobacteria and Firmicutes.At the genus level,the dominant bacteria mainly included three categories:1)photosynthetic bacteria of efficient water purification,such as Rhodomicrobium,Rhodopseudomonas,Rubrivivax,Rhodoblastus and Rhodobacter;2)organic pollutant-degrading bacteria population,including Comamonas,Variovorax,Acinetobacter and Acidovorax;3)denitrifying bacteria population,which joined denitrification and nitrogen cycle,such as Variovorax,Comamonas,Desulfovibrio,Rhodobacter,Acinetobacter,Mesorhizobium and Rubrivivax.PICRUSt functional prediction showed that bacteria in the biofilm had strongest functions in carbohydrate,amino acid and energy metabolism.[Conclusion]The abundance and diversity of bacterial communities in the biofilm under 38μg/L HCB were higher than 760μg/L HCB treatment.In addition,the dominant bacteria at the genus level may be related to eutrophication water purification,organic degradation,denitrification and nitrogen cycle.These results provide a theoretical basis for the removal mechanism of pollutants in eutrophic water.

关 键 词：富营养化水体 六氯苯 狭叶香蒲 生物膜 菌群结构 

分 类 号：X524[环境科学与工程—环境工程]