应用胶体金法检测叶类蔬菜中吡虫啉、多菌灵、啶虫脒、噻虫嗪的残留量分析  

Determination of Imidacloprid,Carbendazim,Acetamiprid and Thiamethoxam Residues in Leafy Vegetables by Colloidal Gold Immunochromatography

作　　者：占绣萍[1] 刘彬 黄兰淇[1] 陈秀[1] 马琳[1] 陈建波[1] 赵莉[1] Zhan Xiuping;Liu Bin;Huang Lanqi;Chen Xiu;Ma Lin;Chen Jianbo;Zhao Li(Shanghai Agriculture Technology Extension&Service Centre,Pesticide Quality Supervision And Inspection Testing Centre(Shanghai),Ministry Of Agriculture,Shanghai 201103,China;Vegetable Technical Extension Station Qingpu District Shanghai,Shanghai 201200,China)

机构地区：[1]上海市农业技术推广服务中心,农业农村部农药质量监督检验测试中心,上海201103 [2]上海市青浦区蔬菜技术推广站,上海201200

出　　处：《农药科学与管理》2021年第10期24-31,共8页Pesticide Science and Administration

基　　金：上海市科委科技创新行动计划19391902300。

摘　　要：研究胶体金法在叶类蔬菜中吡虫啉、啶虫脒、噻虫嗪和多菌灵残留量检测的应用,并将该方法与液相色谱串联质谱法比较,证实胶体金试纸条在检测蔬菜中多菌灵、吡虫啉和啶虫脒残留的适用性。胶体金法检测蔬菜中吡虫啉、啶虫脒、噻虫嗪和多菌灵的检出限分别为0.05、0.05、0.01和0.25mg/kg;该方法在6种叶类蔬菜中的稳定性较好;实验室加标验证4种农药残留检测的假阴性率和假阳性率均为0,与参比方法无显著性差异(χ^(2)<3.84);实物阳性样品验证4种农药残留检测的假阴性率均为0,假阳性率分别为0、0、5.0%和0,但与参比仪器方法均无显著性差异(χ^(2)<3.84)。The application of colloidal gold method for the determination of Imidacloprid,Acetamiprid,thiamethoxam and Carbendazim residues in leafy vegetables was investigated,and the results showed that the colloidal gold strip was suitable for the determination of Carbendazim,imidacloprid and Acetamiprid residues in vegetables.The detection limits of Imidacloprid,Acetamiprid,thiamethoxam and Carbendazim in vegetables were 0.05,0.05,0.01 and 0.25 mg/kg,respectively by Colloidal gold method;the stability of the method was good in the six leafy vegetables;the false negative rate and false positive rate of the four pesticide residues were verified to be 0 by laboratory spiking.which were not significantly different from those of the reference method(X^(2)<3.84);and the false negative rate of the four pesticide residues in the positive samples was 0 and the false positive rates were 0,0,5.0%and 0,respectively,but there was no significant difference with the reference instrument method(X^(2)<3.84).

关 键 词：胶体金法 叶类蔬菜 农药 残留量 

分 类 号：S482[农业科学—农药学] S481.8[农业科学—植物保护]