海洋球石藻(Emiliania huxleyi)活性物质分离鉴定及其抗菌抗氧化性能研究  

ISOLATION AND ANTIMICROBIAL AND ANTIOXIDANT PROPERTIES OF BIO-ACTIVE COMPOUNDS FROM MARINE COCCOLITHOPHORES EMILLIANIA HUXLEYI

作　　者：刘静雯[1] 吴书燕 马亚瑞[1] 张恩权 李桂玲[1] 李健[1] LIU Jing-Wen;WU Shu-Yan;MA Ya-Rui;ZHANG En-Quan;LI Gui-Ling;LI Jian(College of Marine Food and Biological Engineering,Jimei University,Xiamen 361021,China)

机构地区：[1]集美大学海洋食品与生物工程学院,厦门361021

出　　处：《海洋与湖沼》2021年第5期1180-1190,共11页Oceanologia Et Limnologia Sinica

基　　金：国家自然科学基金项目,42076086号,41576166号;福建省自然科学基金项目,2019J01696号。

摘　　要：以海洋球石藻Emiliania huxleyi为原料,提取分离生物活性物质,为该藻活性物质的研究与开发提供理论基础。通过对球石藻75%乙醇浸提物采用乙酸乙酯进行萃取,进一步利用薄层色谱、硅胶色谱、Sephadex LH-20凝胶色谱及高效液相色谱(HPLC)等方法,对化合物成分进行逐步分离纯化,获得纯度大于95%的单体化合物;通过^(1)HNMR、^(13)CNMR及HRMS等波谱分析技术鉴定化合物的结构,并对其抗菌、抗氧化性能进行初步分析。结果分离鉴定出3种单体化合物,分别为叶绿醇(C_(20)H_(40)O)、棕榈酸(C_(16)H_(32)O_(2))和肉豆蔻酸(C_(14)H_(28)O_(2)),这些化合物均为首次从海洋球石藻中分离获得。其中叶绿醇具有较强的抗氧化能力,且对肝癌细胞Hep G2的增殖有较强的抑制作用,其他两种化合物对金黄色葡萄球菌、枯草芽孢杆菌及副溶血性弧菌均有不同程度的抗菌活性。另外,海洋球石藻中这些化合物除了具有一定的抗菌抗氧化性能外,可能在球石藻响应胁迫(如病毒感染)过程中也具有重要作用。To evaluate the antimicrobial and antioxidant activities and the chemical composition of marine coccolithophores Emilliania huxleyi,the 75%-ethanol extract of E.huxleyi was purified with ethyl acetate,and then separated by thin layer chromatography(TLC),silica gel,Sephadex LH-20,and HPLC chromatography.By spectroscopic analysis including nuclear magnetic resonance(NMR)and mass spectrometer(MS),three compounds in high-purity of 95%were identified on chemical structures.The three compounds were phytol,palmitic acid,and myristic acid,and they were isolated from E.huxleyi for the first time.Phytol has a stronger antioxidant activities and inhibitory activities against human Hep G2 cell line.Palmitic acid and myristic acid exhibit different antibacterial activities against Bacillus subtilis,Staphylococcus aureus,and Vibrio parahemolyticus.In addition to the antibacterial and antioxidant activities,these compounds may also play an important role in response to environmental stress(e.g.,virus infection),calling for more studies in the future.

关 键 词：海洋球石藻Emiliania huxleyi 化学成分 结构鉴定 抗菌抗氧化活性 

分 类 号：Q946[生物学—植物学]