反溶剂法制备玉米醇溶蛋白-海藻酸钠复合微粒研究  

Study on Zein-sodium Alginate Composite Particles by Anti-solvent Method

作　　者：安丰豪 曹煜婕 姜珊珊 刘佶轩 李明 AN Feng-hao;CAO Yu-jie;JIANG Shan-shan;LIU Ji-xuan;LI Ming(College of Food Science and Engineering,Tonghua Normal University,Tonghua 134001,China)

机构地区：[1]通化师范学院食品科学与工程学院,吉林通化134001

出　　处：《中国果菜》2021年第11期26-30,35,共6页China Fruit & Vegetable

基　　金：通化师范学院大学生创新创业训练计划项目(s202010202044)。

摘　　要：以玉米醇溶蛋白和海藻酸钠为原料,采用反应溶液法制备复合微粒。通过稳定性检测和红外光谱分析,考察了不同制备方法对复合微粒形成及特性的影响。研究结果表明,不同制备方法(F1~F5)对复合微粒的pH、离子强度和热处理稳定性均有显著的影响,其中,F1得到的复合微粒pH和热处理的稳定性相对较好,在pH 3~8的范围内,F1的透光度在45~49范围内变动,变化不显著;在45~100℃的热处理范围内,F1的透光度在43~45范围内变化,变化也不显著。本研究通过对样品透光度的检测间接考察了微粒的粒度大小,结果显示,不同制备方法所得复合微粒的粒度大小为F3<F1<F4<F2<F5。红外光谱结果显示,在1000~1500 cm^(-1)和2000~4000 cm^(-1)范围内,F5的红外光谱与其它方法的红外光谱明显不同,这说明分阶段制备法(F5)与其它制备方法在微粒的形成机制上有本质区别。The composite particles were prepared by anti-solvent method using zein and sodium alginate as materials.The effects of different preparation methods on the formation and properties of the composite particles were investigated by stability testing and infrared spectroscopy analysis.The results showed that the different preparation methods(F1-F5)had significant effects on the pH,ionic strength and heat treatment stability of the particles,among which,F1 had relatively better stability to pH and heat treatment.In pH 3-8,the transmittance of F1 varied in the range of 45 to 49,but the variation was not significant.In 45-100℃heat treatment,the transmittance of F1 varied in the range of 43 to 45 and the variation was not significant.In this study,the particle size of the particles was investigated indirectly through the detection of sample transmittance,and the results showed that the particle sizes of the composite particles obtained by different preparation methods were F3<F1<F4<F2<F5.The results of infrared spectra showed that the infrared spectra of F5 in the range of 1000-1500 cm^(-1) and 2000-4000 cm^(-1) were significantly different from those of other methods,indicating that the phased preparation method(F5)was fundamentally different from other methods in terms of particle formation mechanism.

关 键 词：玉米醇溶蛋白 海藻酸钠 复合微粒 反溶剂法 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]