基于叶片显微结构分析2个核桃品种耐冷胁迫研究  

Study on cold resistance of two walnut varieties based on leaf microstructure

作　　者：刘凯 冯启科 刘晓敏 王红霞 安秀红 张志华 LIU Kai;FENG Qike;LIU Xiaomin;WANG Hongxia;AN Xiuhong;ZHANG Zhihua(College of Horticulture,Hebei Agricultural University,Baoding 071001,China;Hebei Agricultural TechnologyInnovation Center,National Engineering Research Center for Agriculture in Northern Mountainous Areas,Baoding 071001,China)

机构地区：[1]河北农业大学园艺学院,河北保定071001 [2]河北省山区工程创新中心/国家北方山区农业工程技术研究中心,河北保定071001

出　　处：《河北农业大学学报》2021年第6期45-50,共6页Journal of Hebei Agricultural University

基　　金：国家重点研发计划项目(2019YFD1001603);河北省自然科学基金资助项目(C2019204270);现代种业科技创新专项-核桃优异多抗种质创新与新品种选育(21326352D);河北省在读研究生创新项目(CXZZBS2021043).

摘　　要：采用石蜡制片法,对‘清香’和‘辽宁8号’2个品种冷胁迫不同时间下的叶片显微结构进行观察,通过分析叶片表皮厚度、栅栏组织、栅海比、CTR等指标,了解冷胁迫对核桃叶片组织结构的影响,旨在为耐冷胁迫品种选育及响应机制分子解析提供理论依据。结果表明:正常情况下‘辽宁8号’的叶片厚度、表皮厚度、栅栏组织厚度、CTR均大于‘清香’。冷胁迫会破坏细胞结构,使叶片厚度、表皮厚度、细胞结构疏松度均呈现先增加后下降趋势,细胞紧密度则相反,呈先下降后上升趋势。以细胞就紧密度和细胞疏松度为评价标准,‘辽宁8号’耐冷胁迫能力强于‘清香’。The leaf microstructure of two cultivars‘Qingxiang’and‘Liaoning No.8’under cold stress at different times was observed by paraffin sectioning method.The effects of chilling stress on the leaf microstructure of walnut were understood by analyzing the leaf epidermis thickness,palisade tissue,palisade-sea ratio,CTR and other indicators,so as to provide a theoretical basis for the selection of chilling-tolerant cultivars and molecular analysis of response mechanism.The results showed that the leaf thickness,epidermal thickness,palisade tissue thickness and CTR of‘Liaoning NO.8’were higher than those of‘Qingxiang’under normal conditions.Chilling stress can destroy the cell structure,making the leaf thickness,epidermis thickness and cell structure porosity increase first and then decrease,while the cell density is opposite,showing a trend of decrease first and then increase.The chilling tolerance of‘Liaoning NO.8’was stronger than that of‘Qingxiang’according to cell density and porosity.

关 键 词：核桃 显微结构 冷胁迫 

分 类 号：S664.1[农业科学—果树学]