糖肾宝对高糖诱导小鼠肾小球内皮细胞Fli-1、MCP-1及ICAM-1表达的影响  

Effects of Tangshenbao on Fli-1,MCP-1 and ICAM-1 expression of glomerular endothelial cells induced by high glucose in mice

作　　者：向少伟[1] 黄柳慧 陈宇 胡维[1] 黄露[1] 龙韵[1] 贺西南[1] 许雯雯 XIANG Shao-wei;HUANG Liu-hui;CHEN Yu;HU Wei;HUANG Lu;LONG Yun;HE Xi-nan;XU Wen-wen(Department of Nephrology,Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine,Nanning 530011,China;Guangxi University of Chinese Medicine,Nanning 530001,China)

机构地区：[1]广西中医药大学附属瑞康医院肾内科,南宁530011 [2]广西中医药大学,南宁530001

出　　处：《中华中医药杂志》2021年第10期5854-5858,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81573906)。

摘　　要：目的:探讨中药复方糖肾宝对高糖环境下肾小球内皮细胞迁移功能、Fli-1及炎症因子表达的影响。方法:合成Fli-1-siRNA并转染小鼠肾小球内皮细胞。体外培养小鼠肾小球内皮细胞分为正常组(正常血清)、高糖组(30mmol/L葡萄糖+正常血清)、高糖+糖肾宝血清组(30mmol/L葡萄糖+糖肾宝血清)、Fli-1-siRNA组(30mmol/L葡萄糖+Fli-1-siRNA+正常血清)、Fli-1-siRNA+糖肾宝组(30mmol/L葡萄糖+Fli-1-siRNA+糖肾宝血清)、空转组(30mmol/L葡萄糖+Control-siRNA+正常血清)。实时定量PCR检测Fli-1、MCP-1、ICAM-1 mRNA表达水平;Western blot法检测Fli-1、MCP-1、ICAM-1蛋白表达水平;Transwell检测小鼠肾小球内皮细胞迁移率。结果:与正常组比较,高糖诱导各组内皮细胞迁移率显著下降(P<0.01);与高糖组比较,高糖+糖肾宝组、Fli-1-siRNA组和Fli-1-siRNA+糖肾宝组内皮细胞迁移率显著升高(P<0.01)。与正常组比较,高糖组、高糖+糖肾宝组及空转组Fli-1、MCP-1、ICAM-1 mRNA及蛋白表达水平均显著升高(P<0.01,P<0.05);与高糖组比较,高糖+糖肾宝组、Fli-1-siRNA组和Fli-1-siRNA+糖肾宝组Fli-1、MCP-1、ICAM-1 mRNA及蛋白表达水平显著下降(P<0.05,P<0.01)。结论:中药复方糖肾宝可能通过抑制高糖培养小鼠肾小球内皮细胞Fli-1的表达,减少炎症因子的产生,改善内皮细胞迁移率。Objective:To investigate the effect of Tangshenbao on the migration function of glomerular endothelial cells,Fli-1 and the expression of inflammatory factors in high glucose environment.Methods:Fli-1-siRNA was synthesized and transfected into mouse glomerular endothelial cells.The cultured mouse glomerular endothelial cells in vitro were divided into:normal group(normal serum),high glucose group(30 mmol/L glucose+normal serum),high glucose+Tangshenbao serum group(30 mmol/L glucose+Tangshenbao serum),Fli-1-siRNA group(30 mmol/L glucose+Fli-1-siRNA+normal serum),Fli-1-siRNA+Tangshenbao group(30 mmol/L glucose+Fli-1-siRNA+Tangshenbao serum),the mock group(30 mmol/L glucose+control siRNA+normal serum).The expression levels of Fli-1,MCP-1 and ICAM-1 mRNA detected by Real-time quantitative PCR,the protein levels of Fli-1,MCP-1 and ICAM-1 were detected by Western blot,and the migration rate of glomerular endothelial cells was detected by Transwell.Results:Compared with the normal group,the cell mobility in each group induced by high glucose decreased(P<0.01).Compared with the high glucose group,the cell mobility increased in the high glucose+Tangshenbao group,Fli-1-siRNA group and Fli-1-siRNA+Tangshenbao group(P<0.01).Compared with the normal group,the expression levels of Fli-1,MCP-1 and ICAM-1 mRNA and protein in the high glucose group,the high glucose+Tangshenbao group and the mock group were significantly increased(P<0.01,P<0.05).Compared with high glucose group,the expression levels of Fli-1,MCP-1 and ICAM-1 mRNA and protein in the high glucose+Tangshenbao group,the Fli-1-siRNA group and the Fli-1-siRNA+Tangshenbao group were significantly lower(P<0.05,P<0.01).Conclusion:Tangshenbao may reduce the production of inflammatory factors and improve the migration rate of endothelial cells by inhibiting the expression of Fli-1 in high glucose culture.

关 键 词：糖肾宝 糖尿病肾病 肾小球内皮细胞 FLI-1 炎症反应 

分 类 号：R285.5[医药卫生—中药学]