灵芝饮片标准汤剂制备及质量分析方法  

Study on preparation and quality analysis of standard decoction of Ganoderma

作　　者：郭中原[1,2] 郝单丽 谢冉 杨米一 刘丽[1] 宋亚芳[3] 马海 GUO Zhong-yuan;HAO Dan-li;XIE Ran;YANG Mi-yi;LIU Li;SONG Ya-fang;MA Hai(Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;College of Pharmacy,Henan University of Chinese Medicine,Zhengzhou 450008,China;Yanjing Medical College,Capital Medical University,Beijing 101300,China)

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]河南中医药大学药学院,郑州450008 [3]首都医科大学燕京医学院,北京101300

出　　处：《中华中医药杂志》2021年第10期6178-6183,共6页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：中国中医科学院中药研究所、上海市药材有限公司、日本国株式会社津村科研合作项目(No.H2016021-14)。

摘　　要：目的:以pH值、出膏率、转移率、指标成分含量及指纹图谱为参数对灵芝饮片标准汤剂进行研究并确立质量评价方法,为灵芝饮片标准汤剂规范化及其配方颗粒的制备提供依据。方法:采用紫外分光光度法(UV法)测定其多糖、三萜及甾醇的含量,参照传统煎药工艺制备标准汤剂,计算出膏率及转移率,采用高效液相色谱法研究指纹图谱。结果:12批灵芝饮片标准汤剂中含多糖以葡萄糖(C_(6)H_(12)O_(6))计>0.78mg/mL,三萜及甾醇以齐墩果酸(C_(30)H_(48)O_(3))计>0.23mg/mL;pH值为4.0~4.8,多糖转移率范围为27.6%~75.5%;三萜及甾醇转移率范围为9.4%~21.9%,出膏率范围为3.7%~5.7%。通过对12批灵芝指纹图谱研究,有9个共有峰,确认1个色谱峰为参照峰,即9号色谱峰。结论:建立的制备工艺符合传统汤剂制备方法且稳定可行,可用于灵芝饮片标准汤剂研究及质量评价。Objective:To evaluate the pH value,extraction rate,transfer rate,content determination and feature maps or fingerprints by establishing the standard decoction of Ganoderma,and to provide references for the standardization of Ganoderma standard decoction and the preparation of Ganoderma dispensing granules.Methods:The standard decoction of Ganoderma was prepared based on the traditional decoction process.The content of polysaccharides,triterpene and sterols were determined by Ultraviolet Spectrophotometry(UV),the extraction rate,the transfer rate and the fingerprint were evaluated by HPLC.Results:According to the measurement of 12 batches of Ganoderma standard decoction,the content of glucose was greater than 0.78 mg/mL,the content of oleanolic acid was greater than 0.23 mg/mL,the pH value was 4.0 to 4.8,the transfer rate of polysaccharides ranged from 27.6%to 75.5%,the transfer rate of triterpene and sterol ranged from 9.4%to 21.9%,and extraction rate of triterpene and sterol was in the range of 3.7%~4.7%.Through the study on the fingerprint of 12 batches of Ganoderma,nine peaks were confirmed in this research and the peak 9 was confirmed as reference peak.Conclusion:The preparation method established conforms to the traditional decoction method is stable and feasible,which can be used to the research and quality evaluation of standard decoction of Ganoderma.

关 键 词：灵芝 标准汤剂 转移率 出膏率 指纹图谱 

分 类 号：R283.6[医药卫生—中药学]