有氧运动联合螺旋藻营养补充对2型糖尿病大鼠学习记忆的影响及其机制  

The effects of aerobic exercise and spirulina supplement on learning and memory function in hippocampus of type 2 diabetes rats and its mechanism

作　　者：朱洪竹[1] 朱梅菊[1] ZHU Hong-zhu;ZHU Mei-ju(School of PE&Sports Science,Jinggangshan University,Jian 343009,China)

机构地区：[1]井冈山大学体育学院,吉安343009

出　　处：《天然产物研究与开发》2021年第11期1845-1853,共9页Natural Product Research and Development

基　　金：国家自然科学基金(31660291,31360255);江西省自然科学基金(20202BABL206124);江西省教育厅科技计划(GJJ190562)。

摘　　要：本文采用2型糖尿病大鼠模型,应用Tunel荧光染色、Western blot等方法研究有氧运动联合螺旋藻对2型糖尿病大鼠学习记忆功能和对ERK/CREB信号通路及cleaved caspase-3表达的影响。成模大鼠随机分为4组:糖尿病模型组(DM)、糖尿病+运动组(DE)、糖尿病+螺旋藻组(DS)、糖尿病+运动+螺旋藻组(DES),另设对照组(C),每组10只。实验末,检测海马p-ERK、p-CREB、ERK、CREB、cleaved caspase-3蛋白表达,观察海马细胞凋亡情况,评估大鼠学习记忆能力,同时测试血糖、血胰岛素等指标。结果显示:与DM组相比,DE、DS、DES组血糖和血胰岛素水平均降低(P<0.05或P<0.01),逃避潜伏期明显缩短(P<0.05或P<0.01),穿越平台次数增多(P<0.05或P<0.01),海马细胞凋亡率及cleaved caspase-3表达均明显减少(P<0.05或P<0.01),p-ERK/ERK与p-CREB/CREB水平均明显增加(P<0.01)。其中以DES组的改善效果最好。以上说明有氧运动和螺旋藻能降低血糖,有效改善糖尿病大鼠学习记忆,且运动联合螺旋藻方案有更明显的应用效果,优于单纯的有氧运动和螺旋藻,其机理可能与其进一步上调ERK/CREB信号途径,减少cleaved caspase-3表达,一定程度抑制细胞凋亡,发挥海马神经元保护作用有关。Using the rat model of type 2 diabetes and the methods of Tunnel dyeing and Western blot,this study aimed to investigate effects of aerobic exercise plus spirulina supplement(SP)on learning and memory function,ERK/CREB signaling pathway and cleaved caspase-3 expression in hippocampus of type 2 diabetes rats.The model rats were randomly divided into diabetes model group(DM),diabetes exercise group(DE),diabetes SP group(DS)and diabetes exercise SP group(DES),additionally provided control group(C)without any intervention(10 rats each group).Learning and memory and the hippocampus cells apoptosis rates were observed.The expressions of p-ERK,p-CREB,ERK,CREB and cleaved caspase-3 were tested;at the same time,changes of blood glucose and serum insulin,and so on were examined in the end of the experiment.Results:Compared with group DM,blood glucose and serum insulin levels of the intervention groups decreased significantly(P<0.05 or P<0.01),the escape latencies of the groups were shortened and the numbers plateau crossing increased(P<0.05 or P<0.01).The cells apoptosis rate and cleaved caspase-3 expression of the rats decreased significantly(P<0.05 or P<0.01),the levels of P-ERK/ERK,P-CREB/CREB in the groups increased obviously(P<0.01).The improvement of the above indicators was more obvious in group DES than that in group DE and DS.These results suggested that aerobic exercise and SP could reduce blood glucose and improve learning and memory of type 2 diabetes rats,and the neuroprotective effect of exercise combined with SP was better than those in exercise and SP alone.The mechanism is related to the up-regulation of ERK/CREB signaling pathway,and down-regulation of cleaved caspase-3 expression then further inhibit hippocampus cell apoptosis for type 2 diabetes rats.

关 键 词：糖尿病 学习记忆 有氧运动 螺旋藻 ERK/CREB信号通路 细胞凋亡 

分 类 号：R131[医药卫生—劳动卫生]