青阳参根中一个新的苯二氢化萘型木脂素  

A new phenyldihydronaphthalene lignan from the root of Cynanchum otophyllum Schneid

作　　者：李小叁 龙娟 罗宇浩 何妙莹 吴绮靖 刘立 杨雪梅 LI Xiao-san;LONG Juan;LUO Yu-hao;HE Miao-ying;WU Qi-jing;LIU Li;YANG Xue-mei(School of Pharmacy,Guangdong Medical University,Dongguan 523808,China)

机构地区：[1]广东医科大学药学院,东莞523808

出　　处：《天然产物研究与开发》2021年第11期1860-1865,共6页Natural Product Research and Development

基　　金：广东省粤莞联合基金(2019A1515110386);湛江市科技发展专项资金竞争性分配项目(2020A01029);广东省中医药局面上科研项目(20211228);广东省医学科研基金(A2021218)。

摘　　要：为了解青阳参(Cynanchum otophyllum Schneid)根的酸催化水解物化学成分。利用硅胶柱色谱、ODS、HPLC等多种柱色谱分离手段对青阳参95%乙醇提取物的酸催化水解物进行了分离纯化,从中得到了4个木脂素,通过波谱学方法鉴定其结构,分别为9′-methoxynegundin B(1)、(+)-isolariciresinol(2)、dehydroconiferyl alcohol(3)、5-methoxydehydroconiferyl alcohol(4)。其中化合物1为新的苯二氢化萘型木脂素,化合物2~4为首次从本属植物中分离得到。采用MTT法测定了化合物1~4对人癌细胞株HeLa、H1299、HepG2和MCF-7的细胞毒活性,结果表明化合物2和3对肝癌HepG2细胞有明显的抑制活性。To understand the chemical constituents from the acid catalytic hydrolysate of the root of Cynanchum otophyllum Schneid.Four lignan compounds were isolated from the acid catalytic hydrolysate of the 95%ethanol extract of the root of C.otophyllum by means of multiple columnchromatography techniques including silica gel,ODS and preparative HPLC.Their structures were elucidated by spectroscopic analysis and comparison of their spectroscopic data with those reported in the literature to be 9′-methoxynegundin B(1),(+)-isolariciresinol(2),dehydroconiferyl alcohol(3),5-methoxydehydroconiferyl alcohol(4).Among them,1 was new compound and compounds 2-4 were obtained for the first time in the genus Cynanchum.The cytotoxicities of compounds 1-4 toward four human carcinoma cell lines,HeLa,H1299,HepG2,and MCF-7 cells were evaluated by MTT method.The results showed that compounds 2 and 3 had strong inhibitory effects on the growth of HepG2 carcinoma cell line.

关 键 词：青阳参 木脂素 结构鉴定 细胞毒活性 

分 类 号：R932[医药卫生—生药学]