辣木叶对糖尿病大鼠认知功能及海马神经细胞凋亡的影响  

Effects of spicy leaves on cognitive function and apoptosis of hippocampal neurons in diabetic rats

作　　者：孙杨[1] 赵灿 刘媛媛[1] 隋月林[1] 朱建忠[1] SUN Yang;ZHAO Can;LIU Yuan-yuan;SUI Yue-lin;ZHU Jian-zhong(Cangzhou Medical College,Department of Anatomy,Cangzhou 061000,China)

机构地区：[1]沧州医学高等专科学校解剖教研室,河北沧州061000

出　　处：《中国应用生理学杂志》2021年第6期638-643,共6页Chinese Journal of Applied Physiology

基　　金：国家卫生计生委计划生育与优生重点实验室开放性课题(20150002);河北省科技厅指令课题(0827610D-20)。

摘　　要：目的:探讨辣木叶对链脲佐菌素(STZ)诱导的糖尿病模型大鼠认知功能障碍及海马神经细胞凋亡的影响。方法:选取健康SD雄性大鼠50只,随机选取10只作为对照组,40只大鼠给予腹腔注射25 mg/kg STZ构建糖尿病大鼠模型。40只大鼠随机分为模型组、辣木高、中、低剂量组,分别每日灌胃给药2.0 g/kg、4.0 g/kg、8.0 g/kg辣木叶,对照组和模型组每日灌胃给药等量生理盐水,1次/日,持续给药8周。Morris水迷宫实验评价大鼠学习记忆能力;HE染色及免疫组化染色对大鼠海马神经元切片进行染色,观察各组大鼠海马神经元病理改变的情况及Bax、caspase-3及Bcl-2蛋白表达情况;酶联免疫法(ELISA)检测大鼠海马组织中肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)。结果:与对照组相比,模型组大鼠血糖值明显提高(P<0.01),血胰岛素水平明显降低(P<0.05);与模型组相比,辣木组血糖值显著降低(P<0.05,P<0.01),辣木中、高剂量组血胰岛素水平显著升高(P<0.05,P<0.01)。不同剂量组辣木组间比较,给药后3组大鼠FBG及INS无统计学差异(P>0.05);Morris水迷宫实验的第4-5日,与模型组相比较,辣木各剂量组潜伏期均明显缩短(P<0.05);辣木不同剂量组目标象限停留时间明显延长(P<0.05)。炎症因子变化结果,与模型组相比较,辣木低、中、高剂量组TNF-α、IL-6含量及蛋白表达水平显著降低(P<0.05)。HE染色及免疫组化染色结果显示,与模型组相比较,辣木中剂量组中阳性表达,呈棕黄色,细颗粒状。辣木叶中剂量组(53.21±7.19)能显著减少神经元细胞凋亡的数目(P<0.01);辣木组中剂量组大鼠海马组织中Bax、caspase-3的蛋白表达及Bax/Bcl-2比例显著降低(P<0.05)。结论:辣木叶能改善糖尿病大鼠认知功能障碍其作用机制可能与调节Bax、Bcl-2、caspase-3的蛋白表达,降低炎症因子TNF-α及IL-6含量相关。Objective:To investigate the effects of Moringa leaves on the cognitive dysfunction and apoptosis of hippocampal neurons in diabetic rats induced by streptozotocin(STZ).Methods:Fifty male SD rats were selected,and 10 rats were randomly selected as the control group.The other 40 rats were treated with STZ at the dose of 25 mg/kg by intraperitoneal injection.The 40 diabetic rats were randomly divided into model group,Moringa oleifera low-dose,medium-dose and high-dose group.The rats in Moringa oleifera groups were treated with Moringa oleifera at the doses of 2.0,4.0 and 8.0 g/kg by gavage,the control group and model group were treated with the same amount of normal saline once a day,for 8 weeks.Morris water maze test was used to evaluate the learning and memory ability of rats.Pathological changes of hippocampal neurons and expressions of Bax,caspase-3 and bcl-2 protein in each group were observed by the sections were stained with HE staining and immunohistochemistry.Enzyme linked immunosorbent assay(ELISA)was used to detect tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)in rat.Results:compared with the control group,the blood glucose of the model group was increased significantly(P<0.01),and the blood insulin level was decreased significantly(P<0.05);compared with the model group,the blood glucose values of Moringa oleifera groups were decreased significantly(P<0.05,P<0.01),and the blood insulin levels of middle and high dose Moringa oleifera group were increased significantly(P<0.05,P<0.01).There was no significant difference in FBG and INS among the three groups(P>0.05).In Morris water maze test,compared with the model group,the latency of Moringa oleifera groups was significantly shorter(P<0.05);the residence time in target quadrant of Moringa oleifera groups with different doses was significantly prolonged(P<0.05).Compared with the model group,the contents of TNF-α,IL-6 and protein expression in low,medium and high dose groups of Moringa oleifera were decreased significantly(P<0.05).HE staining and i

关 键 词：辣木叶 糖尿病 认知功能 炎症因子 

分 类 号：R285.5[医药卫生—中药学]