桦木酸对胃癌SGC-7901细胞凋亡的影响  

Effects of Betulinic acid on apoptosis in human gastric cancer SGC-7901 cells

作　　者：丁璐 邵淑丽[1,2] 何孟奇 黄鑫 张伟伟[1,2] 张珍珠[1,2] DING Lu;SHAO Shu-li;HE Meng-qi;HUANG Xin;ZHANG Wei-wei;ZHANG Zhen-zhu(College of Life Science and Agriculture and Forestry,Qiqihar University,Qiqihaer 161000,China;Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas,Qiqihar University,Qiqihaer 161000,China)

机构地区：[1]齐齐哈尔大学生命科学与农林学院 [2]齐齐哈尔大学抗性基因工程与寒地生物多样性保护黑龙江省重点实验室,黑龙江齐齐哈尔161006

出　　处：《中国应用生理学杂志》2021年第5期495-499,547,共6页Chinese Journal of Applied Physiology

基　　金：齐齐哈尔大学黑龙江省教育厅基本业务专项重点项目(135109104);黑龙江省高教强省优势特色学科—玉米“粮头食尾”重点专项(LTSW201737);黑龙江省省属高等学校基本科研业务费科研项目(植物性食品加工技术特色学科专项YSTSXK201809)。

摘　　要：目的:以人胃癌SGC-7901细胞为研究对象,探究桦木酸对其凋亡的影响。方法:将人胃癌SGC-7901细胞分为4组,每组设置3个复孔,对照组未加入桦木酸,而三组实验组分别加入浓度为10 mg/L、20 mg/L及30 mg/L的桦木酸,将各组细胞放入5%的CO 2培养箱中培养48 h,激光共聚焦显微镜观察细胞形态变化;流式细胞术检测细胞凋亡率和线粒体膜电位变化;qRT-PCR和Western blot分别检测SGC-7901细胞凋亡相关基因Bcl-2、Bax和Caspase-3在mRNA和蛋白水平的表达。结果:与对照组相比,终浓度为10 mg/L、20 mg/L、30 mg/L的桦木酸处理组,细胞发生皱缩、细胞核裂解并出现凋亡小体;细胞早期凋亡与晚期凋亡率显著增加(P<0.05 or P<0.01),线粒体膜电位明显降低(P<0.05 or P<0.01);细胞凋亡相关基因Bax和Caspase-3的mRNA与蛋白表达水平均显著上升(P<0.01),而Bcl-2的mRNA与蛋白表达水平显著降低(P<0.01)。结论:在一定浓度范围内,桦木酸通过调节凋亡相关基因Bcl-2、Bax和Caspase-3的表达诱导人胃癌SGC-7901细胞凋亡。Objective:To investigate the effects of betulinic acid on apoptosis of human gastric cancer SGC-7901 cells.Methods:The human gastric cancer SGC-7901cells were divided in to 4 groups,and each group was set with 3 replicates.The SGC-7901cells in control group were not treated with betulinic acid;the other 3 experimental groups were treated with betulinic acid at the concentrations of 10,20 and 30 mg/L,respectively;each group was incubated in a 5%carbon dioxide incubator for 48 h.Laser confocal microscope was used to observe morphological changes of SGC-7901 cells;Flow cytometry was applied to determine apoptosis rate and mitochondrial membrane potential.The mRNA and protein levels of Bcl-2,Bax and Caspase-3 were also detected by qRT-PCR and western blot respectively.Results:Compared with the control group,SGC-7901 cells in the treated group at final concentrations of 10,20 and 30 mg/L shrinked,appeared apoptosis body along with nuclear splitting.The percentage of cells in early and advanced period of apoptosis were markedly increased(P<0.05 or P<0.01),mitochondrial membrane potential was obviously reduced(P<0.05 or P<0.01).qRT-PCR and western blot analysis showed that the mRNA and protein expressions of Bax and Caspase-3 were increased significantly(P<0.01),while the expressions of Bcl-2 were decreased significantly(P<0.01).Conclusion:Within a certain range of concentrations,betulinic acid induces cell apoptosis by regulating the expression of Bcl-2,Bax and Caspase-3 in human gastric cancer.

关 键 词：桦木酸 人胃癌SGC-7901细胞 细胞凋亡 

分 类 号：Q256[生物学—细胞生物学]