pVAX-GHRH表达质粒对D-半乳糖诱导老年动物骨质疏松的影响  

Effect of pVAX-GHRH expression plasmid on D-galactose-induced osteoporosis in elderly animals

作　　者：叶睿 周浩 陈婷 孙加节 习欠云 张永亮 YE Rui;ZHOU Hao;CHEN Ting;SUN Jiajie;XI Qianyun;ZHANG Yongliang(National Engineering Research Center for Breeding Sxvine Industry&Key Laboratory of Animal Nutrition Control in Guangdong,College of Animal Science,South China Agricultural University,Guangzhou 510642,China)

机构地区：[1]华南农业大学动物科学学院,国家生猪种业工程技术研究中心,广东省动物营养调控重点实验室,广东广州510642

出　　处：《中国兽医学报》2021年第11期2182-2189,2195,共9页Chinese Journal of Veterinary Science

基　　金：国家重大基础研究计划资助项目(2016-YFD0500-503);国家自然科学基金资助项目(31802156);广东省自然科学基金资助项目(c1914050000046)。

摘　　要：将40只小鼠随机分为对照组、GHRH质粒组、D-半乳糖造模+GHRH质粒组、D-半乳糖造模组。采用颈背部皮下注射D-半乳糖(每天500 mg/kg)构建衰老模型,非造模组颈部皮下注射等体积的生理盐水。在造模第3周,分别按80μg/kg注射pVAX空质粒或pVAX-GHRH表达质粒。在质粒注射后5周,测定结果表明,注射GHRH表达质粒能够显著增加血清中GHRH和IGF-Ⅰ的含量,增加骨小梁面积占比,提高骨量。结果表明,GHRH表达质粒注射后能够改善D-半乳糖导致的动物骨质疏松。This study aims to explore the effect of injection of pVAX-GHRH expression plasmid into leg muscles on D-galactose-induced osteoporosis.Forty mice were randomly divided into the control group,GHRH plasmid group,D-galactose model+GHRH plasmid group,and D-galactose model group,10 mice in each group.500 mg/(kg·d^(-1))D-galactose was injected subcutaneously on the back of neck to construct an aging model,and an equal volume of normal saline was injected subcutaneously into the neck of the control group.On the third week of modeling,the pVAX empty plasmid or pVAX-GHRH expression plasmid was injected at the dosage of 80μg/kg.Five weeks after the injection,the results showed that the injection of the GHRH expression plasmid could significantly increase the content of serum GHRH and IGF-Ⅰand increase the proportion of trabecular bone area,improve bone mass.Our results indicate that injection of GHRH expression plasmid can play a role in improving osteoporosis in elderly animals.

关 键 词：GHRH表达质粒 D-半乳糖 骨质疏松 

分 类 号：S852.23[农业科学—基础兽医学]