Initiation of Acupoint Molecular Mechanisms for Manual Acupuncture Analgesia—Nuclear FactorκB Signaling Pathway  

作　　者：ZHANG Kuo ZHAO Xue YANG Tao ZHANG Yan-fang YAN Ya-wen XU Zhi-fang GONG Yi-nan LIU Yang-yang GUO Yong-ming GUO Yi 

机构地区：[1]Academy of Medical Engineering and Translational Medicine,Tianjin University,Tianjin(300072),China [2]Research Center of Experimental Acupuncture Science,Tianjin University of Traditional Chinese Medicine,Tianjin(301617),China [3]Affiliated Hospital of Municipal Institute of Traditional Chinese Medicine of Changzhi City,Shanxi Province(046000),China [4]Sanhuai Road Community Health Service Center,Tanggu Street,Binhai New Area,Tianjin(300451),China [5]School of Traditional Chinese Medicine,Tianjin University of Traditional Chinese Medicine,Tianjin(301617),China

出　　处：《Chinese Journal of Integrative Medicine》2021年第12期933-939,共7页中国结合医学杂志（英文版）

基　　金：the China Postdoctoral Science Foundation(No.2019M651044);the National Natural Science Foundation of China(No.81330088)。

摘　　要：Objective To identify the prominent molecular signaling in acupoints and explore their roles in initiating the analgesia effect of manual acupuncture(MA).Method A three-step study was conducted,the experiment 1 was a genome-wide analysis of the tissue at acupoint Zusanli(ST 36),including 12 Wistar rats which were divided into control,control+MA1,and control+MA7 groups.In the experiment 2,the paw withdrawal latency(PWL),immunohistochemistry and Western blot analysis of phospho-nuclear factor kappa B(NFκB)p65(p-p65),phospho-NFκB p50(p-p50)at ST 36 were performed on rats of saline,saline+MA,and complete Freund’s adjuvant(CFA)+MA groups(n=6).In experiment 3,24 rats were divided into saline+DMSO,CFA+DMSO,CFA+DMSO+MA,and CFA+BAY 11–7082+MA groups,the PWL and immunofluorescence assay of NFκB p65 at ST 36 was conducted.Result(1)The gene:inhibitor of NFκB(Nfkbia),interleukin-1β(Il1b),interleukin-6(Il6),chemokine c-x-c motif ligand 1(Cxcl1),monocyte chemoattractant protein-1(MCP-1/Ccl2)expressions in the control+MA7 group were significantly increased(P<0.05 or P<0.01),and the expression of NFκB p65(Rela),NFκB p50(Nfkb1)were increased in the control+MA7 group(P<0.05).(2)CFA+MA groups showed increased PWL from day 1 to 7(P<0.01 vs.CFA),and the Western blot results were consistent with immunohistochemistry,the expression of NFκB p-p65 and NFκB p-p50 were significantly increased in the MA-related groups compared with control and CFA groups(P<0.05).(3)Compared with the CFA+DMSO+MA group,the PWL of the CFA+BAY 11–7082+MA group decreased significantly and continued until day 5 and 7(P<0.05 and P<0.01,respectively),and the NFκB p65 expression of CFA+BAY 11–7082+MA was significantly reduced compared with CFA+DMSO+MA(P<0.01).Conclusion Local NFκB signaling cascade in acupoint caused by MA is an important step in initiating the analgesic effect,which would provide new evidence for the initiation of MA-effect and improve the understanding of the scientific basis of acupuncture analgesia.

关 键 词：manual acupuncture ACUPOINT ANALGESIA nuclear factor kappa B 

分 类 号：R245[医药卫生—针灸推拿学]