穿龙薯蓣皂苷元对DBA1/J小鼠CD4^(+)CD25^(-)T细胞的影响  

Effects of Diosgenin on CD4^(+)CD25^(-)T Cell in Spleen of DBA1/J Mice

作　　者：梁秀军[1] 董永彩[2] 李强[1] 陈萌[1] 田焕娜[1] 赵晓菲[1] LIANG Xiu-jun;DONG Yong-cai;LI Qiang;CHEN Meng;TIAN Huan-na;ZHAO Xiao-fei(Chengde Medical University,Chengde,Hebei,067000,China;Staff Hospital of Chengde Iron and Steel Company Ltd,Chengde,Hebei,067000,China)

机构地区：[1]承德医学院,河北承德067000 [2]承德钢铁集团有限公司职工医院

出　　处：《承德医学院学报》2021年第6期463-466,共4页Journal of Chengde Medical University

基　　金：河北省高等学校科学技术研究项目(QN2016031);河北省中医药管理局科研计划项目(2015155)。

摘　　要：目的初步探讨穿龙薯蓣皂苷元对DBA1/J小鼠CD4^(+)CD25^(-)T细胞的影响。方法采用MACS磁珠分选法分离DBA1/J小鼠脾CD4^(+)CD25^(-)T细胞,并鉴定纯度。穿龙薯蓣皂苷元作用CD4^(+)CD25^(-)T细胞后,采用ELISA方法检测各组培养上清中IL-10的浓度,Real-Time PCR方法检测Foxp3的水平,FCM检测Treg细胞的比率。结果免疫磁珠术分选DBA1/J小鼠脾CD4^(+)CD25^(-)T细胞纯度达93.89%。给药作用48h后,与对照组相比,穿龙薯蓣皂苷元组上清中IL-10的浓度显著升高(P<0.05),Foxp3 mRNA水平明显上升(P<0.05),Treg细胞比率明显升高(P<0.05)。结论穿龙薯蓣皂苷元能明显提升DBA1/J小鼠脾脏CD4^(+)CD25^(-)T细胞上清中细胞因子IL-10浓度,Treg细胞Foxp3 mRNA水平及Treg细胞比率。Objective To investigate the effect of diosgenin on CD4^(+)CD25^(-)T cells of DBA1/J mice.Methods CD4^(+)CD25^(-)T cells of DBA1/J mice were separated with immunomagnetic beads.Then,CD4^(+)CD25^(-)T cell purity was detected by fl ow cytometry and culture them in vitro.After treated with diosgenin,the concentration of IL-10 was detected by ELISA,the level of transcription factor Foxp3 mRNA was detected by real-time PCR,and the proportion of Treg cells was detected by FCM.Results This study separated CD4^(+)CD25^(-)T cells of 93.89%purity.Compared with the control group,the concentration of IL-10 in cell supernatant,the Foxp3 mRNA level and percentage of Treg cells were upregulated signifififi cantly(P<0.05)after treated with diosgenin for 48h.Conclusion Diosgenin can signififi cantly upregulate the levels of IL-10 and Foxp3 mRNA,the proportion of Treg cells in DBA1/J mice.

关 键 词：穿龙薯蓣皂苷元 CD4^(+)CD25^(-)T细胞 调节性T细胞 白细胞介素-10 FOXP3 

分 类 号：R285.5[医药卫生—中药学]