小麦-中间偃麦草2A/6St代换系014-459的分子细胞遗传学鉴定  

Molecular cytogenetic identification of wheat-Thinopyrum intermedium 2A/6St substitution strain 014-459

作　　者：陶军[1,2] 兰秀锦 TAO Jun;LAN Xiu-Jin(Triticeae Research Institute,Sichuan Agricultural University,Chengdu 611130,Sichuan,China;Mianyang Academy of Agricultural Sciences,Mianyang 621023,Sichuan,China)

机构地区：[1]四川农业大学小麦研究所,四川成都611130 [2]绵阳市农业科学研究院,四川绵阳621023

出　　处：《作物学报》2022年第2期511-517,共7页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(31970243)资助。

摘　　要：中间偃麦草是小麦遗传改良的有用资源,育成了大量的小麦-中间偃麦草附加系、代换系及部分双二倍体。中4是小麦-中间偃麦草部分双二倍体,很方便与普通小麦杂交并被广泛用于小麦的遗传改良。014-459是中4与普通小麦杂交后代,具有一些特殊特性,材料014-459与一些普通小麦杂交,无论正反交,其F1表现为不育,而与另一些普通小麦的杂交F1表现为可育,此外,材料014-459粗蛋白和湿面筋含量很高。基于014-459的这些特性,猜测其可能具有中间偃麦草染色体片段,为此对014-459进行了细胞学鉴定。FISH和GISH以及PLUG标记分析用来分析材料014-459的染色体组成情况。连续的FISH和GISH试验证实小麦-中间偃麦草部分双二部体中4与小麦杂交后代品系014-459的1对小麦2A染色体被来自中间偃麦草的1对St染色体代换,PLUG标记分析证实这1对St染色体属于第6同源群,可能来自中间偃麦草的St染色体被代换进小麦中造成了材料014-459的一些特性。对品系014-459的分子细胞遗传学鉴定对促进中间偃麦草6St染色体在小麦中利用及小麦品质改良有积极作用。Thinopyrum intermedium is a valuable resource for wheat genetic improvement,and numerous wheat-Th.intermedium chromosome addition and substitution lines and partial amphiploids have been developed.Zhong 4 is a wheat-Th.intermedium partial amphidiploid,which can easily hybridize with wheat and is extensively utilized for wheat improvement.The strain 014-459 was a progeny of partial amphidiploid Zhong 4 descendant of wheat and Th.intermedium with some special characteristics such as high crude protein and wet gluten content and partial F1 of common wheat cultivars and strain 014-459 were sterility while a few others were fertility despite reciprocal cross.Molecular cytogenetic identification of strain 014-459 were detected for speculated contained fragment of Th.intermedium chromosomes based on its special characters.Genomic composition of strain 014-459 was detected by FISH,GISH,and PLUG(polymerase chain reaction-based landmark unique gene) marker analysis.Combined FISH/GISH analysis confirmed that a pair of wheat 2 A chromosomes in strain 014-459 of the hybrid progeny between wheat-Thinopyrum intermedium partial amphidiploid Zhong 4 and wheat was replaced by a pair of St chromosomes from Thinopyrum intermedium.PLUG marker analysis verified that this pair belonged to the sixth homologous group.The special characters of strain 014-459 might be attributable to the substitution of Th.intermedium St chromosome into wheat.The study of molecular cytogenetic identification of strain 014-459 was probably beneficial to quality improvement and utilization of 6 St chromosome of Th.intermedium in wheat breeding.

关 键 词：GISH FISH 代换系 拟鹅冠草 细胞遗传学 

分 类 号：S512.1[农业科学—作物学]