脾多肽注射液对治疗原发性血小板减少症的作用途径研究  

Effect of lienal polypeptide injection on primary immune thrombocytopenia

作　　者：岳昭蓉 谢飞 王欣 李红玉 YUE Zhaorong;XIE Fei;WANG Xin;LI Hongyu(Institute of Microbiology,School of Life Sciences,Lanzhou University,Lanzhou 730000,Gansu,China;School of Pharmacy,Lanzhou University,Lanzhou 730000,Gansu,China)

机构地区：[1]兰州大学生命科学学院微生物学研究所,甘肃兰州730000 [2]兰州大学药学院,甘肃兰州730000

出　　处：《中国临床药理学与治疗学》2021年第11期1229-1236,共8页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：甘肃省自然科学基金项目(20JR10RA602)。

摘　　要：目的:探索脾多肽注射液(LPI)对原发性血小板减少症(ITP)模型小鼠血小板生成的影响。方法:用随机数表法将实验用SPF级昆明小鼠进行分组,小鼠隔天腹腔注射兔抗鼠血小板血清(APS)诱导构建小鼠ITP模型。在设定时间通过尾静脉方式采血,检测各组血小板、红细胞、白细胞的数量变化,同时计算胸腺、脾脏指数,通过骨髓涂片的方式对巨核细胞进行分类并计数,最后用ELISA法检测血清中促血小板生成素(TPO)、干细胞因子(SCF)、白介素-3(IL-3)、白介素-6(IL-6)、白介素-11(IL-11)、β-血小板球蛋白(β-TG)、转化生长因子-β1(TGF-β1)、血小板因子-4(PF-4)表达水平。结果:LPI可显著提高ITP模型小鼠血小板计数水平,对红细胞、白细胞无影响;胸腺、脾脏指数显著下降;小鼠骨髓中的原始巨核细胞、产血小板巨核细胞数升高,幼稚型巨核细胞、颗粒型巨核细胞和裸核型巨核细胞数均无显著变化;血清中细胞因子SCF、IL-3、IL-6显著升高,PF-4显著降低。结论:LPI可能通过上调IL-3、IL-6、SCF水平和下调PF-4水平来提高ITP小鼠的血小板数量,对LPI的临床推广应用具有指导意义。AIM:To explore the effect of lienal polypeptide injection(LPI)on platelet production in ITP model mice with primary immune thrombocytopenia.METHODS:SPF Kunming mice were divided into groups by random number table method,and the ITP model of mice was induced by intraperitoneally injected of rabbit anti-mouse platelet serum APS every other day.At the set time,blood was collected through caudal vein to detect the changes in the number of platelets,red blood cells and white blood cells in each group.Meanwhile,the thymus and spleen indexes were calculated.Megakaryocytes were classified and counted by means of bone marrow smear.Finally,the expression levels of TPO,SCF,IL-3,IL-6,IL-11,β-TG,TGF-β1 and PF-4 in serum were detected by ELISA.RESULTS:LPI significantly increased platelets count in ITP model mice,but had no effect on red blood cells and white blood cells.Thymus and spleen index decreased significantly.The number of megakaryoblasts and thrombocytopenia megakaryocytes in the bone marrow of mice increased,but the number of promegakaryocytes,granular megakaryocytes and naked megakaryocyte did not change significantly.Serum sytokines SCF,IL-3 and IL-6 were significantly increased,while PF-4 was decreased significantly.CONCLUSION:LPI may increase the platelet count in ITP mice by up regulating the levels of IL-3,IL-6,SCF and down regulating the level of PF-4,which has guiding significance for clinical promotion application of LPI.

关 键 词：脾多肽注射液 原发性血小板减少症 血小板 红细胞 巨核细胞 

分 类 号：R965.2[医药卫生—药理学]