Single-cell RNA sequencing of meiocytes and microspores reveals the involvement of the Rf4 gene in redox homeostasis of CMS-C maize  

作　　者：Huaisheng Zhang Huili Yang Desheng Hu Bing Li Yanan Lin Wen Yao Zhanyong Guo Haochuan Li Dong Ding Zhanhui Zhang Yanmin Hu Yadong Xue Jihua Tang 

机构地区：[1]National Key Laboratory of Wheat and Maize Crop Science,College of Agronomy,Henan Agricultural University,Zhengzhou 450002,Henan,China [2]College of Life Sciences,Henan Agricultural University,Zhengzhou 450002,Henan,China

出　　处：《The Crop Journal》2021年第6期1237-1247,共11页作物学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (31571745 and 31971893);the Key Technology Research and Development Program of Henan Province (202102110164 and 212102110061);the Zhengzhou Major Science and Technology Innovation Project (188PCXZX803);the Open Funds of the State Key Laboratory of Crop Genetics and Germplasm Enhancement (ZW202001)。

摘　　要：Normal microsporogenesis is determined by both nuclear and mitochondrial genes. In maize C-type cytoplasmic male sterility, it is unclear how the development of meiocytes and microspores is affected by the mitochondrial sterility gene and the nuclear restorer gene. In this study, we sequenced the transcriptomes of single meiocytes(tetrad stage) and early mononucleate microspores from sterile and restorer lines. The numbers of expressed genes varied in individual cells and fewer than half of the expressed genes were common to the same cell types. Four comparisons revealed 3379 differentially expressed genes(DEGs), with 277 putatively associated with mitochondria, 226 encoding transcription factors,and 467 possibly targeted by RF4. KEGG analysis indicated that the DEGs in the two lines at the tetrad stage were involved predominantly in carbon metabolism and in amino acid biosynthesis and metabolism, whereas the DEGs during the transition from the tetrad stage to the early mononucleate stage were associated mostly with regulation of protein metabolism, fatty acid metabolism, and anatomical structure morphogenesis. Thus, meiocyte and microspore development was affected by the surrounding cells and the restorer gene, and the restorer gene helped restore the redox homeostasis of microspores and the normal cellular reconstruction during the transition.

关 键 词：MAIZE Single-cell sequencing Cytoplasmic male sterility Restoration HETEROSIS 

分 类 号：S513[农业科学—作物学]