Arsenite provides a selective signal that coordinates arsenate uptake and detoxification through the regulation of PHR1 stability in Arabidopsis  

作　　者：Cristina Navarro Cristian Mateo-Elizalde Thotegowdanapalya C.Mohan Eduardo Sanchez-Bermejo Oscar Urrutia Maria Nieves Fernandez-Muniz Jose M.Garcia-Mina Riansares Munoz Javier Paz-Ares Gabriel Castrillo Antonio Leyva 

机构地区：[1]Department of Plant Molecular Genetics,Centro Nacional de Biotecnología-Consejo Superior de Investigaciones Científicas,Madrid 28049,Spain [2]Department of Environmental Biology,Sciences School,University of Navarra,Pamplona 31008,Spain [3]Department of Analytical Chemistry,School of Chemical Sciences,Universidad Complutense de Madrid,Madrid 28040,Spain [4]Present address:Department of Biotechnology and Bioinformatics,Faculty of Life Sciences,JSS Academy of Higher Education and Research,Mysuru,Karnataka 570015,India [5]Present address:S&A Fresh Produce.Brook Farm,Marden,Hereford HR13ET,UK [6]Future Food Beacon of Excellence&School of Biosciences,University of Nottingham,Nottingham LE125RD,UK

出　　处：《Molecular Plant》2021年第9期1489-1507,共19页分子植物（英文版）

基　　金：This work was supported by fellowships from the Spanish Ministry of Science and Innovation(MICINN)to C.N.,C.M.-E.,E.S.-B.and G.C.and by afellowship from Severo Ochoa Centres of Excellence Grant Programme to C.N.and from La Caixa/CNB International PhD fellowship to T.C.M.Thiswork was funded by Spanish Ministry of Science and lnnovation GrantsBIO2014-55741-R,BIO2017-87524-R and BIO2017-89530;by the Com-mission of Science and Technology Grant CTQ2017-83569-C2-1-R;andby the Comunidad of Madrid and European funding from FSE and FEDERprograms Grants S2018/BAA-4393 and AVANSECAL-Il-CM.

摘　　要：In nature,plants acquire nutrients from soils to sustain growth,and at the same time,they need to avoid the uptake of toxic compounds and/or possess tolerance systems to cope with them.This is particularly challenging when the toxic compound and the nutrient are chemically similar,as in the case of phosphate and arsenate.In this study,we demonstrated that regulatory elements of the phosphate starvation response(PSR)coordinate the arsenate detoxification machinery in the cell.We showed that arsenate repression of the phosphate transporter PHT1;1 is associated with the degradation of the PSR master regulator PHR1.Once arsenic is sequestered into the vacuole,PHR1 stability is restored and PHT1;1 expression is recovered.Furthermore,we identified an arsenite responsive SKP1-like protein and a PHR1 interactor F-box(PHIF1)as constituents of the SCF complex responsible for PHR1 degradation.We found that arsenite,the form to which arsenate is reduced for compartmentalization in vacuoles,represses PHT1;1 expression,providing a highly selective signal versus phosphate to control PHT1;1 expression in response to arsenate.Collectively,our results provide molecular insights into a sensing mechanism that regulates arsenate/phosphate uptake depending on the plant’s detoxification capacity.

关 键 词：arsenic signaling F-box protein arsenate uptake arsenic detoxification phosphate starvation PHYTOREMEDIATION 

分 类 号：Q94[生物学—植物学]