DNA demethylases remodel DNA methylation in rice gametes and zygote and are required for reproduction  

作　　者：Shaoli Zhou Xue Li Qian Liu Yu Zhao Wei Jiang Anqi Wu Dao-Xiu Zhou 

机构地区：[1]National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University,430070 Wuhan,China [2]Institute of Plant Science Paris–Saclay(IPS2),CNRS,INRAE,University Paris–Saclay,91405 Orsay,France

出　　处：《Molecular Plant》2021年第9期1569-1583,共15页分子植物（英文版）

基　　金：This work was supported by grants from the National Key Research and Development Program of China(2016YFD0100802);the National Natural Science Foundation of China(31730049);Huazhong Agricultural University Scientific&Technological Self-innovation Foundation(program 2016RC003);Fundamental Research Funds for the Central Universities(2662015PY228);National Postdoctoral Program for Innovative Talents.

摘　　要：Fertilization constitutes a critical step in the plant life cycle during which the gamete genomes undergo chromatin dynamics in preparation for embryogenesis. In mammals, parental chromatin is extensively reprogrammed through the global erasure of DNA methylation. However, in flowering plants it remains unclear whether and how DNA methylation is remodeled in gametes and after fertilization in the zygote. In this study, we characterize DNA methylation patterns and investigate the function of DNA glycosylases in rice eggs, sperm, and unicellular zygotes and during embryogenesis. We found that DNA methylation is locally reconfigured after fertilization and is intensified during embryogenesis. Genetic, epigenomic, and transcriptomic analysis revealed that three rice DNA glycosylases, DNG702, DNG701, and DNG704, demethylate DNA at distinct genomic regions in the gametes and the zygote, and are required for zygotic gene expression and development. Collectively, these results indicate that active DNA demethylation takes place in the gametes and the zygote to locally remodel DNA methylation, which is critical for egg and zygote gene expression and reproduction in rice.

关 键 词：DNA methylation DNA glycosylase FERTILIZATION 

分 类 号：S511[农业科学—作物学]