An Enzyme-Free Amperometric Sensor Based on Self-Assembling Ferrocene-Conjugated Oligopeptide for Specific Determination of L-Arginine  

作　　者：Kai-Jie Zhu Li Zhou Ling Wu Sai-Fei Feng Hui-Ying Hu Jing-Lin He Yu-Min He Ze-Meng Feng Yu-Long Yin Donghong Yu Zhong Cao 

机构地区：[1]Hunan Provincial Key Laboratory of Materials Protection for Electric Power and Transportation,and Hunan Provincial Key Laboratory of Cytochemistry,School of Chemistry and Biological Engineering,Changsha University of Science and Technology,Changsha,Hunan,410114 China [2]Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha,Hunan 410125,China [3]Department of Chemistry and Bioscience,Aalborg University,DK-9220 Aalborg,East,Denmark

出　　处：《Chinese Journal of Chemistry》2021年第10期2755-2762,共8页中国化学（英文版）

基　　金：supported by the projects of the National Natural Science Foundation of China(Nos.31527803,21545010,21275022 and 21645009);the Natural Science Foundation of Hunan Province,China(Nos.2020JJ4599,2019JJ50651).

摘　　要：Main observation and conclusion An enzyme-free amperometric sensor based on a heptadecapeptide possessing an electroactive ferrocene(Fc)linker as ferrocene-Gly-Gly-Gly-Gly-Phe-Gly-His-Ile-His-Glu-Gly-Tyr-Gly-Gly-Gly-Gly-Lys-(CH_(2))_(4)-dithiocyclopentane self-assembled on gold substrate was designed and fabricated for specific determination of L-arginine(L-Arg).The detection mechanism is based on conformational change of surface-immobilized peptide induced by the target L-Arg,which was confirmed via SEM,TEM,AFM,XPS,and SPR studies.The binding affinity and the recognition feasibility of immobilized specific and non-specific peptides were also assessed using electrochemical impedance spectroscopy(EIS),cyclic voltammetry(CV),and differential pulse voltammetry(DPV).The proposed method can serve as“signal-on”sensor for detection of L-Arg down to 31 pmol/L with broad linear range(0.0001 to 10μmol/L).Furthermore,the Fc-conjugated specific peptide sensor was successfully applied to the determination of L-Arg in pig serums with a recovery rate of 97.5%—106.9%,and its test results are in good agreement with that of chromatographic instrument,evidencing that the oligopeptide-based sensor can be served as a simple and enzyme-free biosensing platform towards L-Arg for future application.

关 键 词：L-ARGININE Peptides Surface Analysis Enzyme-Free Amperometric Sensor 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置]