Confined Electrochemiluminescence at Microtube Electrode Ensembles for Local Sensing of Single Cells  

作　　者：Hao Ding Weiliang Guo Lurong Ding Bin Su 

机构地区：[1]Institute of Analytical Chemistry,Department of Chemistry,Zhejiang University,Hangzhou,Zhejiang,310058 China [2]School of Chemistry and Materials Science,Nanjing Normal University,Nanjing,Jiangsu 210023,China

出　　处：《Chinese Journal of Chemistry》2021年第10期2911-2916,共6页中国化学（英文版）

基　　金：supported by the National Natural Science Foundation of China(21904115,22074131,21874117);the Natural Science Foundation of Zhejiang Province(LZ18B050001);the China Postdoctoral Science Foundation(2020T130577,2019M662019).

摘　　要：Main observation and conclusion Chemical analysis of single cells plays essential roles in both fundamental research and clinical applications.In this work,the electrochemiluminescence(ECL)generated at vertically aligned gold microtube electrode ensembles(MEEs)was applied for the local chemical sensing of single cells.Thanks to the structure of MEEs and the surface-confined nature of ECL generation,the ECL is confined in single microtubes and the local variations of hydrogen peroxide concentration can be revealed by the changes of ECL signals at single MEEs.With this methodology,we studied the hydrogen peroxide efflux from single living cells,showing the subcellular heterogeneity in hydrogen peroxide release.With sufficient spatial resolution for the parallel single cell sensing as well as advantages including easy preparation and high throughput,the MEEs provide a promising platform for subcellular electroanalysis.

关 键 词：ELECTROCHEMILUMINESCENCE Single cells ELECTROCHEMISTRY Microarrays Sensors 

分 类 号：O657.1[理学—分析化学]