仿细胞膜结构聚合物涂层对醋酸纤维素膜透析器生物相容性的影响  

Effect of cell membrane mimetic polymer coating on biocompatibility of cellulose acetate membrane dialyzer

作　　者：史珂慧[1] 王彦兵 高菊林[1] 张欢 宫永宽[4] 蒋红利[1] SHI Kehui;WANG Yanbing;GAO Julin;ZHANG Huan;GONG Yongkuan;JIANG Hongli(Dialysis Department of Nephrology Hospital,First Affiliated Hospital of Xi'an Jiaotong University,Xi'an 710061,China;School of Textile Science and Engineering,Xi'an Polytechnic University,Xi'an 710048,China;Key Laboratory of Functional Textile Material and Product(Xi'an Polytechnic University),Ministry of Education,Xi'an 710048,China;Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education,College of Chemistry and Materials Science and Institute of Materials Science and New Technology,Northwest University,Xi'an 710127,China)

机构地区：[1]西安交通大学第一附属医院肾脏病医院血液净化科,西安710061 [2]西安工程大学纺织科学与工程学院 [3]西安工程大学功能性纺织材料及制品教育部重点实验室 [4]西北大学化学与材料科学学院合成与天然功能分子教育部重点实验室

出　　处：《肾脏病与透析肾移植杂志》2021年第5期425-430,共6页Chinese Journal of Nephrology,Dialysis & Transplantation

基　　金：国家自然科学基金(81870507);陕西省自然科学基础研究计划一般项目(2021JQ⁃665)。

摘　　要：目的:研究仿细胞膜结构聚合物2甲基丙烯酰氧乙基磷酰胆碱/对硝基苯氧羰基聚乙二醇甲基丙烯酸酯/多巴胺(PMNC)涂层对醋酸纤维素膜(CA)透析器生物相容性的影响。方法:将PMNC聚合物涂敷在CA膜表面,通过体外蛋白质吸附实验和动物体外循环(ECC)实验观察膜表面对纤维蛋白原吸附、血小板、补体和白细胞的黏附及激活的作用。结果:与无涂层CA膜相比,PMNC聚合物涂层CA膜对牛血清白蛋白和纤维蛋白原的吸附量分别下降了81%和82%。在ECC过程中,无涂层组血小板、白细胞计数及纤维蛋白原浓度均出现明显下降,同时β血小板球蛋白、白细胞介素8、凝血酶⁃抗凝血酶复合物、补体片段5a和凝血酶原片段1+2浓度显著上升,而PMNC聚合物涂层组上述指标均没有明显变化。ECC 120 min后无涂层组透析器凝血现象明显,而PMNC聚合物涂层组透析器均未发生凝血。结论:PMNC聚合物涂层能够显著提高CA膜透析器的生物相容性,本研究为进一步将PMNC聚合物涂层应用于医疗器械的表面改性提供了重要的实验依据。Objective:To study the effect of cell membrane mimetic polymer PMNC coating on blood compatibility of dialyzer.Methodology:PMNC polymer was coated on the surface of cellulose acetate(CA)membrane.The effects of membrane surface on fibrinogen(Fib)adsorption,adhesion and activation of platelets(PLT),complement and leukocytes were observed by in vitro protein adsorption and animal extracorporeal circulation(ECC).Results:Compared with the uncoated CA membrane,the adsorption of serum bovine albumin and fibrinogen on the PMNC polymer coated CA membrane decreased by 81%and 82%,respectively.During the animal ECC,the PLT and WBC counts and the FIB concentrations in the uncoated dialyzer group decreased and the concentrations ofβ⁃TG,IL⁃8,C5a,TAT and F1+2 increased significantly.However,those parameters did not significant change during ECC through the PMNC coated dialyzers.After ECC 120 min,the coagulation of dialyzer was obvious in the uncoated group but no coagulation occured in the PMNC polymer group.Conclusion:These results suggest that PMNC polymer coating could significantly improve biocompatibility of CA membrane dialyzer,which provides an important experimental basis for further applying PMNC polymer coating to the surface modification of medical devices.

关 键 词：透析器 血液相容性 醋酸纤维素膜 磷脂聚合物 仿贻贝结构 

分 类 号：R318.08[医药卫生—生物医学工程]