乳疾康纳米微乳制剂治疗乳腺增生小鼠的药效学研究  

Pharmacodynamic Study on Rujikang Naoemulsion Preparation in the Treatment of Hyperplasia of Mammary Glands in Mice

作　　者：张弦[1] 赵强[1] 徐巧玲[1] 丁一 李霞[1] 唐华非[1] 马瑞[1] ZHANG Xian;ZHAO Qiang;XU Qiaoling;DING Yi;LI Xia;TANG Huafei;MA Rui(Dept.of Pharmacy,the 305th Hospital of PLA,Beijing 100017,China;Dept.of Pharmacy,Xijing Hospital of Air Force Medical University,Shaanxi Xi’an 710032,China)

机构地区：[1]解放军第305医院药局,北京100017 [2]空军军医大学西京医院药学部,陕西西安710032

出　　处：《中国医院用药评价与分析》2021年第11期1286-1290,1297,共6页Evaluation and Analysis of Drug-use in Hospitals of China

基　　金：国家自然科学基金项目(No.81603385)。

摘　　要：目的:对乳疾康纳米微乳制剂治疗乳腺增生小鼠的药效学进行研究。方法:将ICR小鼠随机分为正常组、模型组和治疗组[溴隐亭组(涂抹溴隐亭)、高剂量组(涂抹高剂量乳疾康纳米微乳制剂)和低剂量组(涂抹低剂量乳疾康纳米微乳制剂)]。建立乳腺增生小鼠模型,对治疗组小鼠进行药物干预30 d后,观察体重、乳晕和乳头直径的变化,检测血清雌二醇(E_(2))和孕酮(P)水平,采用苏木精-伊红染色法观察乳房组织病理结构变化,采用TUNEL染色法观察组织凋亡细胞情况,采用免疫印迹法检测组织中Caspase-3和Bcl-2蛋白的表达。结果:与模型组比较,治疗组小鼠体重降低(P<0.05),乳晕、乳头直径缩短(P<0.01,P<0.001),E_(2)水平降低(P<0.05),P水平升高(P<0.05),凋亡细胞数量减少(P<0.001),Caspase-3蛋白表达上调(P<0.001),Bcl-2蛋白表达下调(P<0.01),上述差异均有统计学意义。与模型组比较,治疗组小鼠乳腺组织结构和上皮细胞结构基本恢复正常。结论:乳疾康纳米微乳制剂能显著抑制激素紊乱导致的小鼠乳腺增生。OBJECTIVE:To investigate the pharmacodynamics of Rujikang naoemulsion preparation in the treatment of hyperplasia of mammary glands in mice.METHODS:The ICR mice were randomly divided into normal group,model group and treatment group[bromocriptine group(coated with bromocriptine),high-dose group(coated with high-dose Rujikang naoemulsion preparation)and low-dose group(coated with low-dose Rujikang naoemulsion preparation)].The mouse model of mammary gland hyperplasia was established,after 30-day drug intervention on the treatment group,the changes in body mass,areola and nipple diameters were observed,the serum estradiol(E_(2))and progesterone(P)levels were measured,the changes in pathogeny structure of breast tissue were observed by hematoxylin-eosin staining method,the apoptotic cells were observed by TUNEL staining method,the Caspase-3 and Bcl-2 protein expression were detected by Western blot method.RESULTS:Compared with the model group,mice in the treatment group had lower body mass(P<0.05),shorter areola and nipple diameter(P<0.01,P<0.001),lower E_(2) level(P<0.05),higher P level(P<0.05),fewer in number of apoptotic cells(P<0.001),up-regulated Caspase-3 protein expression(P<0.001),and down-regulated Bcl-2 protein expression(P<0.01),differences were statistically significant.Compared with the model group,structures of mammary tissue and epithelial cell of the treatment group were basically restored to normal.CONCLUSIONS:The Rujikang naoemulsion preparation can significantly inhibit hyperplasia of mammary glands in mice caused by hormonal disorder.

关 键 词：乳疾康纳米微乳 乳腺增生 雌二醇 孕酮 Caspase-3 Bcl-2 

分 类 号：R932[医药卫生—生药学] R96[医药卫生—药学]