A special o-dialdehyde fluorescent probe simultaneously sensing Hcy,GSH and its application in living cells and zebrafish imaging  

作　　者：Yongbin Zhang Yu Zhang Yongkang Yue Jianbin Chao Fangjun Huo Caixia Yin 

机构地区：[1]Shanxi Key Laboratory of Functional Molecules,Research Institute of Applied Chemistry,Shanxi University,Taiyuan 030006,China [2]School of Chemistry and Chemical Engineering,Shanxi University,Taiyuan 030006,China [3]Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education,Institute of Molecular Science,Shanxi University,Taiyuan 030006,China [4]Scientific Instrument Center,Shanxi University,Taiyuan 030006,China

出　　处：《Chinese Chemical Letters》2021年第9期2873-2876,共4页中国化学快报（英文版）

基　　金：the National Natural Science Foundation of China(Nos.21672131,21775096,21907062);One Hundred People Plan of Shanxi Province;Shanxi Province"1331 Project"Key Innovation Team Construction Plan Cultivation Team(No.2018-CT-1);2018 Xiangyuan County Solid Waste Comprehensive Utilization Science and Technology Project(No.2018XYSDJS-05);the Shanxi Province Foundation for Selected(2019);Innovative Talents of Higher Education Institutions of Shanxi(2019);Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi(No.2019L0031);Key R&D Program of Shanxi Province(No.201903D421069);Natural Science Foundation of Shanxi Province of China(No.201901D111015);Shanxi Province Foundation for Returness(No.2017-026);Program for the Innovative Talents of Higher Education Institutions of Shanxi(2019);Shanxi Collaborative Innovation Center of High Value-added Utilization of Coalrelated Wastes(No.2015-10-B3);Scientific Instrument Center of Shanxi University(No.201512)。

摘　　要：The development of fluorescent probes enabling to distinguish Cys,Hcy and GSH has always been a considerable challenge,in particular the distinction of Hcy and other two biothiols,because Hcy has a very similar structure with Cys and a relatively lower concentration in living organisms.In this work,a special o-dialdehyde fluorescent probe,quinoline-2,3-dicarboxaldehyde(QDA),has been synthesized and demonstrated superior performance in differentiating detection of Hcy and GSH,which is different from the previous reported o-dialdehyde probes specifically detecting GSH.Furthermore,the probe can selectively distinguish Hcy and GSH from different signal channels in living cells and zebrafish,meaning it has great potential in biological applications.This finding will provide a novel idea for the design of fluorescent probes to distinguish biothiols.

关 键 词：o-Dialdehyde DISTINGUISH HCY GSH BIOIMAGING 

分 类 号：R313[医药卫生—基础医学] O657.3[理学—分析化学]