黄瓜抗寒基因CsRAV2的克隆与序列分析  

Cloning and Sequence Analysis of Cucumber Cold Tolerance Gene CsRAV2

作　　者：李爽 石广丽 陈姗姗[3] 周艳丽[3] 赵春波[3] 李胜男[4] Li Shuang;Shi Guangli;Chen Shanshan;Zhou Yanli;Zhao Chunbo;Li Shengnan(Management Office of Teaching and Scientific Research Base,Jilin Agricultural University,Changchun,130118;College of Life Science,Jilin A-gricultural University,Changchun,130118;College of Horticulture,Jilin Agricultural University,Changchun,130118;College of Advanced Agri-culture and Ecological Environment,Heilongjiang University,Harbin,150080)

机构地区：[1]吉林农业大学教研基地管理处,长春130118 [2]吉林农业大学生命科学学院,长春130118 [3]吉林农业大学园艺学院,长春130118 [4]黑龙江大学现代农业与生态环境学院,哈尔滨150080

出　　处：《分子植物育种》2021年第21期7012-7017,共6页Molecular Plant Breeding

基　　金：吉林省科技发展计划项目(20180412002XH);吉林农业大学省级大学生创新创业训练计划项目共同资助。

摘　　要：中国黄瓜品种繁多,很早就开始选育黄瓜品种,但黄瓜的抗逆性不强,容易产生病虫害和冷害。黄瓜幼苗早春定植在露地或冬春季节在保护地栽培,经常会出现低温冷害的情况,这对黄瓜的早熟和高产会有较大影响。因此,分析和验证冷胁迫相关的基因,探究黄瓜抗低温冷害形成的机理,可以为黄瓜耐低温品种的选育和抗性品种的培育奠定基础。本研究中以吉林地区主要栽培品种‘翠秋20’为试验材料,通过RNA-seq测序技术筛选出一个AP2/EREBP转录因子中RAV亚族的CsRAV2,推测该基因可能参与黄瓜幼苗冷胁迫,采用PCR技术克隆CsRAV2的序列全长,同时通过生物信息学分析比对,了解CsRAV2的结构域与分类,结果表明:CsRAV2编码区序列全长1 122 bp,编码了344 aa,为疏水性蛋白质,无明显信号肽和无跨膜结构,亚细胞定位在细胞核。该研究结果为深入探究CsRAV2基因的功能提供了科学依据。There are many varieties of cucumbers in China, and cucumber varieties have been selected and bred very early, but the resistance of cucumbers is not strong, and they are prone to pests and cold damage. Cucumber seedlings are planted in open fields in early spring or cultivated in protected areas in winter and spring. Low temperature and chilling damage often occur. Low temperature and chilling damage usually have a great impact on the early maturity and high yield of cucumber. The study of the mechanism of cucumber resistance to cold damage can lay a foundation for breeding cucumber varieties resistant to low temperature and cultivars resistant to cold damage. In this study, the main cultivar ’Cuiqiu20’ in Jilin area was used as the test material, and the RAV subfamily CsRAV2 in the AP2/EREBP transcription factor was screened by RNA-seq sequencing technology. It is speculated that this gene may be involved Cucumber seedlings were under cold stress, PCR technology was used to clone the full length sequence of CsRAV2. Meanwhile, a series of bioinformatics analyses were performed on the structural domain and classification of CsRAV2. The results show that the CsRAV2 transcription factor has a full length of 1 122 bp and encodes 344 amino acids. The protein is a hydrophobic protein and has no obvious signal peptide and no transmembrane structure. The subcell is located in the nucleus. The results of this study provides theoretical basis for further exploring the function of CsRAV2 gene.

关 键 词：黄瓜 低温冷害 CsRAV2 基因克隆 

分 类 号：S642.2[农业科学—蔬菜学]