月季R2R3-MYB转录因子全基因组鉴定与分析  

Genome-wide Identification and Analysis of the R2R3-MYB Transcription Factor in Rosa chinensis

作　　者：李谋亮 张晓妮 林胜男 吴全淑 包满珠[1] 傅小鹏[1] Li Mouliang;Zhang Xiaoni;Lin Shengnan;Wu Quanshu;Bao Manzhu;Fu Xiaopeng(Key Laboratory of Horticultural Plant Biology,Ministry of Education,College of Horticulture and Forestry Sciences,Huazhong Agricultural University,Wuhan,430070)

机构地区：[1]华中农业大学园艺林学学院,园艺植物生物学教育部重点实验室,武汉430070

出　　处：《分子植物育种》2021年第21期7018-7029,共12页Molecular Plant Breeding

基　　金：国家重点研发计划项目(2019YFD1000400)资助。

摘　　要：月季是世界著名的观赏植物,具有极高的观赏价值和经济价值。本研究基于月季基因组利用生物信息学对月季R2R3-MYB基因家族进行全面鉴定,并对这些基因的结构、保守域和组织特异表达进行研究,解析月季R2R3-MYB转录因子在月季发育过程中的生物学功能。根据已报道的拟南芥R2R3-MYB基因,利用本地BLAST以及Hmmer工具鉴定月季基因组(Rosa chinensis‘Old blush’)中的R2R3-MYB基因,共鉴定出120个月季R2R3-MYB家族基因。系统进化树分析表明,月季R2R3-MYB家族基因可被分为34个亚组;其家族成员氨基酸序列N端均含有2个不等重复结构域R2和R3;基因结构分析显示,月季R2R3-MYB家族基因含有1~11个外显子;染色体定位发现120个基因分别定位在月季的7条染色体上,并发生8次串联重复事件;通过对基因组相关性分析共发现16对重复基因的R2R3-MYB基因簇。基因表达模式分析表明,120个基因在月季发育中均有表达,根据表达聚类分析可分为6个亚组,每个亚组之间存在表达差异。Rosa chinensis is a commercially important ornamental plant cultivated worldwide with high ornamental and economic value. The objectives of this study were to analyze the structure, conserved domain and evolution of R2R3-MYB by bioinformatics. Besides, it was also to study the expression specificity of family members in different tissues and investigate the biological function of R2R3-MYB genes family in Rosa chinensis. According to the reported R2R3-MYB genes of Arabidopsis thaliana, one hundred and twenty members of R2R3-MYB genes in Rosa chinensis ’Old blush’ were identified by BLAST and Hmmer tool. The phylogenetic tree indicated that these genes were divided into 34 subgroups. The N-terminal of the amino acid sequence of R2R3-MYB family members contains 2 unequal repeat domains R2 and R3. The results of gene structure analysis showed that the R2R3-MYB family genes contain 1 to 11 exons;120 genes were located on 7 chromosomes of rose, and 8 tandem repeat events occurred by chromosome mapping analysis. The results of analysis of genome correlation, a total of 16 pairs of R2R3-MYB gene clusters were found. Based on the analysis of gene expression patterns, the results showed that 120 genes were wildly expressed throughout the development of the rose. What’s more, they could be divided into 6 subgroups, and there were different patterns of expression between each subgroup.

关 键 词：月季 R2R3-MYB家族 生物信息学 表达模式 

分 类 号：S685.12[农业科学—观赏园艺]