高山杜鹃转录组测序及MYB转录因子家族分析  

Analysis of Transcriptome Sequencing and MYB Transcription Factor Family in Rhododendron lapponicum

作　　者：贾新平[1] 刘晓青[1] 邓衍明[1] 李畅[1] 苏家乐[1] Jia Xinping;Liu Xiaoqing;Deng Yanming;Li Chang;Su Jiale(Institute of Leisure Agriculture,Jiangsu Academy of Agricultural Sciences,Nanjing,210014)

机构地区：[1]江苏省农业科学院休闲农业研究所,南京210014

出　　处：《分子植物育种》2021年第21期7037-7047,共11页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31700627);江苏省自然科学基金项目(BK20170607)共同资助。

摘　　要：MYB转录因子是植物中最大的一类转录因子家族,参与调控植物的生长发育、次生代谢、逆境胁迫等生物学过程。目前,关于高山杜鹃MYB转录因子的研究尚未见报道。本研究利用SMRT高通量测序技术对高山杜鹃品种‘富丽金陵’进行转录组测序,得到了15.37 Gb数据,通过去冗余获得75 002条转录本序列。其中,71 155、33 653、30 359条转录本分别在NR、GO、COG数据库中具有匹配信息。基于高山杜鹃转录组数据,鉴定了64个转录因子家族,其中MYB基因有220个。根据结构特性将MYB基因分为4类:1R-MYB、R2R3-MYB、R1R2R3-MYB和4R-MYB,其氨基酸序列包含20种保守元件。进化树分析结果显示,高山杜鹃MYB基因分为28个亚组。研究采用单分子实时测序技术(single molecule real time, SMRT)对高山杜鹃品种‘富丽金陵’转录组进行测序,将获得的转录本序列进行功能注释和分类,对获得的220个MYB基因进行生物信息学进行分析,相关结果具有一定参考意义。MYB transcription factor is the largest family of transcription factors in plants, and they are involved in the regulation of plant growth and development, secondary metabolism, adversity stress and other biological processes. So far, there is no the study on the MYB transcription factor of Rhododendron lapponicum. In this study,the Rhododendron lapponicum variety ’Fuli Jinling’ transcriptome was generated by SMRT sequencing technology. A total of 15.37 Gb data was obtained, and 75 002 transcript sequences were obtained by removing redundancy. 71 155, 33 653 and 30 359 transcripts were assigned to the NR, GO and COG databases, respectively.Based on the transcriptome sequencing data, 64 transcription factor gene families were identified, including 220 MYB genes. According to the structural characteristics, the MYB gene is divided into four categories, including 1R-MYB, R2 R3-MYB, R1 R2 R3-MYB and 4R-MYB. The amino acid sequence of MYB transcription factor contains 20 conserved elements. Phylogenetic analysis showed that the MYB genes of Rhododendron lapponicum could be divided into 28 subclasses. In this study, we first used SMRT sequencing technology to generate the Rhododendron lapponicum transcriptome. In this study, single molecule real time(SMRT) technology was used to sequence the transcriptome of the Rhododendron lapponicum variety ’Fuli Jinling’, and the obtained transcript sequences were functionally annotated and classified, and 220 MYB genes for bioinformatics analysis, these related results have certain reference significance.

关 键 词：高山杜鹃 转录组 MYB转录因子 

分 类 号：S685.21[农业科学—观赏园艺]