帕罗西汀对大鼠创伤后应激障碍模型中缝背核5-羟色胺1A受体表达的影响  

Effect of paroxetine on the expression of 5-hydroxytryptamine 1A receptor in the dorsal raphe nucleus in rat model of post-traumatic stress disorder

作　　者：肖鹏 李燕飞 张建标 XIAO Peng;LI Yan-fei;ZHANG Jian-biao(The First Department of Psychiatry,Shandong Daizhuang Hospital,Jining 272051,Shandong Province,China;The Third Department of Psychiatry,The No.4 People’s Hospital of Nantong,Nantong 226000,Jiangsu Province,China)

机构地区：[1]山东省戴庄医院精神一科,山东济宁272051 [2]南通市第四人民医院精神三科,江苏南通226000

出　　处：《中国临床药理学杂志》2021年第22期3127-3130,共4页The Chinese Journal of Clinical Pharmacology

基　　金：山东省医药卫生科技发展计划基金资助项目(2015WS0418)。

摘　　要：目的研究帕罗西汀对大鼠创伤后应激障碍(PTSD)模型中缝背核5-羟色胺(5-HT)1A受体表达的影响。方法将50只Wistar大鼠随机选取10只设置为对照组,其余40只建立PTSD模型,将建模成功的32只大鼠,随机分为模型组(10只)、低剂量实验组(11只)、高剂量实验组(11只)。低、高剂量实验组分别用帕罗西汀20,40 mg·kg^(-1)灌胃,对照组和模型组灌胃等体积生理盐水,每天1次,持续干预14 d。以糖水消耗实验检测大鼠糖水偏嗜度;以免疫组化染色、蛋白质印迹法检测中缝背核5-HT1A受体表达。结果对照组、模型组和低、高剂量实验组糖水偏嗜度分别为(82.54±7.14)%,(59.93±6.52)%,(69.82±7.01)%,(75.50±7.07)%;免疫组化阳性细胞个数分别为(90.00±12.22),(182.00±25.41),(137.00±19.85),(118.00±20.35)个;5-HT1A受体蛋白表达量分别为0.10±0.01,1.26±0.15,0.71±0.08,0.53±0.06。上述指标,模型组与对照组比较,低剂量实验组与模型组比较,高剂量实验组与低剂量实验组比较,差异均有统计学意义(均P<0.05)。结论帕罗西汀可改善PTSD大鼠抑郁、焦虑样行为,降低中缝背核5-HT1A受体表达。Objective To study the effect of paroxetine on the expression of 5-hydroxytryptamine(5-HT)1A receptor in the dorsal raphe nucleus of a rat model of post-traumatic stress disorder(PTSD).Methods Ten of 50 Wistar rats were randomly selected as control group,and the remaining 40 were established as PTSD model.The 32 rats that were successfully modeled were randomly divided into model group(n=10),low dose experimental group(n=11),high dose experimental group(n=11).The low and high dose experimental groups were given paroxetine 20 and 40 mg·kg^(-1) by gavage,control group and model group were given the equal volume of normal saline,once a day,by gavage,for 14 days.The sugar water consumption test was used to detect the sucrose preference in rats.The immunohistochemistry and Western blot were used to detect the expression of 5-HT1A receptor in the dorsal raphe nucleus.Results The syrup partiality of control group,model group and low,high dose experimental groups were(82.54±7.14)%,(59.93±6.52)%,(69.82±7.01)%,(75.50±7.07)%,the n umber of immunohistochemical positive cells were 90.00±12.22,182.00±25.41,137.00±19.85,118.00±20.35.The expression levels of 5-HT1A receptor proteins were 0.10±0.01,1.26±0.15,0.71±0.08,0.53±0.06.For the above indicators,the differences of control group and model group,model group and low dose experimental group,low dose experimental group and high dose experimental group were statistically significant(all P<0.05).Conclusion Paroxetine can improve depression and anxiety-like behavior in PTSD rats,and reduce the expression of 5-HT1A receptor in the dorsal raphe nucleus.

关 键 词：帕罗西汀 创伤后应激障碍 中缝背核 5-羟色胺1A(5-HT1A)受体 

分 类 号：R971[医药卫生—药品]