Anti-EpCAM functionalized graphene oxide vector for tumor targeted siRNA delivery and cancer therapy  

作　　者：Si Chen Shuang Zhang Yifan Wang Xin Yang Hong Yang Chunying Cui 

机构地区：[1]School of Pharmaceutical Sciences,Capital Medical University,Beijing 100069,China [2]Engineering Research Center of Endogenous Prophylactic of Ministry of Education of China,Beijing 10069,China [3]Beijing Area Major Laboratory of Peptide and Small Molecular Drugs,Beijing 10069,China [4]Yanjing Medical College,Capital Medical University,Beijing 101300,China

出　　处：《Asian Journal of Pharmaceutical Sciences》2021年第5期598-611,共14页亚洲药物制剂科学（英文）

基　　金：supported by the National Natural Science Foundation(81502688);Beijing Natural Science Foundation Program and Scientific Research Key Program of Beijing Municipal Commission of Education(KM201810025019);a basic-clinical key research Grant(16JL72,17JL67)from Capital Medical University;the Importation and Development of High-Caliber Talents Project of Beijing Municipal Institutions(2013–2015);Natural Science Foundation of Capital Medical University(1210020232)。

摘　　要：Graphene oxide(GO) has emerged as a potential drug delivery vector. For siRNA delivery, GO should be modified to endow it with gene delivery ability and targeting effect. However, the cationic materials used previously usually had greater toxicity. In this study, GO was modified with a non-toxicity cationic material(chitosan) and a tumor specific monoclonal antibody(anti-EpCAM) for the delivery of survivin-siRNA(GCE/siRNA). And the vector(GCE) prepared was proved with excellent biosafety and tumor targeting effect. The GCE exhibited superior performance in loading si RNA, maintained stability in different solutions and showed excellent protection effect for survivin-siRNA in vitro. The gene silencing results in vitro showed that the m RNA level and protein level were down-regulated by 48.24% ± 2.50% and 44.12% ± 3.03%, respectively, which was equal with positive control( P > 0.05). It was also demonstrated that GCE/siRNA had a strong antitumor effect in vitro, which was attributed to the efficient antiproliferation, and migration and invasion inhibition effect of GCE/siRNA. The results in vivo indicated that GCE could accumulate siRNA in tumor tissues. The tumor inhibition rate of GCE/siRNA 54.74% ± 5.51% was significantly higher than control 4.87% ±8.49%. Moreover, GCE/siRNA showed no toxicity for blood and main organs, suggesting that it is a biosafety carrier for gene delivery. Taken together, this study provides a novel design strategy for gene delivery system and siRNA formulation.

关 键 词：Graphene oxide siRNA delivery Survivin Anti-EpCAM Gene silencing 

分 类 号：R943[医药卫生—药剂学]