羊流产嗜性衣原体蛋白-蛋白相互作用网络的构建  

Construction of the protein-protein interaction network of Chlamydia abortus

作　　者：刘威[1] 袁芳艳[1] 周丹娜[1] 刘泽文[1] 高婷 郭锐[1] 杨克礼[1] 段正赢[1] 梁婉 田永祥[1] LIU Wei;YUAN Fang-yan;ZHOU Dan-na;LIU Ze-wen;GAO Ting;GUO Rui;YANG Ke-li;DUAN Zheng-ying;LIANG Wan;TIAN Yong-xiang(Institute of Animal Science and Veterinary Medicine,Hubei Academy of Agricultural Science/Hubei Science-observation Experiment Station of Veterinary Medicine and Diagnostic Technology(Ministry of Agriculture and Rural Affairs),Wuhan 430064,China)

机构地区：[1]湖北省农业科学院畜牧兽医研究所/农业农村部兽用药物与诊断技术湖北科学观测实验站,武汉430064

出　　处：《湖北农业科学》2021年第22期124-128,共5页Hubei Agricultural Sciences

基　　金：国家重点研发计划项目(2016YFD0500906);国家自然科学基金项目(315020069);湖北省农业科技创新中心资助项目(2016-620-000-001-026)。

摘　　要：病原菌的相互作用组学研究是揭示潜在信号转导通路和新型药物靶标的有力工具。通过同源蛋白映射的方法构建了羊流产嗜性衣原体(Chlamydia abortus)的蛋白-蛋白相互作用网络。结果表明,所构建的互作网络包含220个蛋白和1276个相互作用关系。通过分析蛋白的互作频率,确定了在羊流产嗜性衣原体中互作频率最高的20个蛋白,主要与转录、翻译、分子伴侣等功能相关。进一步分析发现,伴侣蛋白DnaK、GroEL、DnaJ发生互作的频率较高,能与核糖体蛋白、代谢相关蛋白等多种功能蛋白发生相互作用。若DnaK、GroEL、DnaJ的功能受到抑制,将会对羊流产嗜性衣原体的正常生命活动造成较大的影响。Analysis of the pathogen interactome is a powerfull approach for dissecting potential signal trasducion and new drug targets.A comprehensive protein-protein interaction network for Chlamydia abortus consisting of 220 proteins and 1276 interaction pairs was constructed.Our analysis unraveled 20 proteins with highest interactions in the network,most of them are related to the function of transcription,translation and molecular chaperone.Further analysis showed that the chaperone proteins DnaK,GroEL and DnaJ had a high frequency of interaction,and could interact with multiple functional proteins such as ribosomal proteins and metabolism-related proteins.The results suggested that if the function of DnaK,GroEL and DnaJ were inhibited,the normal life activities of Chlamydia abortus would be greatly affected.Taken together,this study therefore provides valuable clues in exploring new signaling protein and new drug targets.

关 键 词：羊流产嗜性衣原体(Chlamydia abortus) 蛋白相互作用 互作网络 

分 类 号：S852.67[农业科学—基础兽医学]