病毒与宿主蛋白互作研究技术进展  

Progress on Research Technology of Virus and Host Protein Interaction

作　　者：石金凤 丁媛 陈子杨 王琦 徐新悦 周泓名 马一丹 焦国财 李健明[2,3,5] 时坤 杜锐[2,3,4,5] SHI Jin-feng;DING Yuan;CHEN Zi-yang;WANG Qi;XU Xin-yue;ZHOU Hong-ming;MA Yi-dan;JIAO Guo-cai;LI Jian-ming;SHI Kun;DU Rui(College of Animal Science and Technology,Jilin Agricultural University,Changchun,Jilin,130118,China;College of Chinese Medicine Materials,Jilin Agricultural University,Changchun,Jilin,130118,China;Research Lab on Key Technology of Sika Deer Development and Resource Utilization,Changchun,Jilin,130118,China;Key Laboratory of Animal Production and Product Quality and Safety of Ministry of Education,Changchun,Jilin,130118,China;Jilin Province Sika Deer High-efficiency Breeding and Product Development Technology Engineering Research Center,Changchun,Jilin,130118,China)

机构地区：[1]吉林农业大学动物科学技术学院,吉林长春130118 [2]吉林农业大学中药材学院,吉林长春130118 [3]梅花鹿药用资源利用关键技术研究室,吉林长春130118 [4]动物生产及产品质量安全教育部重点实验室,吉林长春130118 [5]吉林省梅花鹿高效养殖和产品开发技术工程研究中心,吉林长春130118

出　　处：《动物医学进展》2021年第12期97-101,共5页Progress In Veterinary Medicine

基　　金：国家自然科学基金项目(31672577)。

摘　　要：蛋白质间的相互作用在预测靶蛋白功能、了解病毒感染宿主的机制和研发新型抗病毒药物方面发挥着重要作用。大多数蛋白质以复合物形式来实现其功能。近年来,已经研发了多种基于生物物理、生物化学或遗传学的方法来辅助蛋白质-蛋白质相互作用(PPI)的研究,丰富了PPI数据,为研究蛋白互作提供了重要依据。每种检测方法在敏感性和特异性方面都有自身的优点和局限性。论文围绕几种经典的蛋白互作研究方法,包括表面等离子体共振、免疫共沉淀、谷胱甘肽转移酶沉淀试验和酵母双杂交等,概述其原理,分析其优点、局限性及在病毒学领域的最新应用,以期为研究人员选择合适的PPI研究技术提供参考。Protein interaction plays a key role in predicting the function of the target protein,understanding the mechanism of virus infecting hosts and researching new antiviral drugs.Most proteins fulfill their phenotypic functions in the form of complexes.In recent years,a variety of methods based on biophysics,biochemistry and genetics have been developed to assist the study of protein-protein interaction(PPI),enriching PPI data and providing important information for studying protein-protein interactions.However,each detection method has its own advantages and limitations in terms of sensitivity and specificity.The thesis discussed several classic protein interaction research methods,including Surface plasmon resonance(SPR),Co-immunoprecipitation(Co-IP),Glutathione S-transferase pull-down(GST pull-down)and Yeast two-hybrid(Y2H),etc,and summarized their principles,analyzed their advantages,limitations,and their latest applications in the field of virology,in order to provide a reference for researchers to choose appropriate PPI research techniques.

关 键 词：蛋白质-蛋白质相互作用 表面等离子体共振 免疫共沉淀 谷胱甘肽转移酶沉淀试验 酵母双杂交 

分 类 号：S852.65[农业科学—基础兽医学] S852.21[农业科学—兽医学]