纸基病原体核酸生物传感器的研究进展  

Research Progress in Paper-based Biosensors for Pathogen Nucleic Acids

作　　者：马漪 齐谢敏 武海萍 马雪萍 初亚男 王雪梅 周国华 MA Yi;QI Xie-Min;WU Hai-Ping;MA Xue-Ping;CHU Ya-Nan;WANG Xue-Mei;ZHOU Guo-Hua(Department of Clinical Pharmacy,Affiliated Jinling Hospital,Medical School of Nanjing University,Nanjing 210002,China;School of Pharmacy,Southern Medical University,Nanjing 210002,China)

机构地区：[1]南京大学医学院附属金陵医院(东部战区总医院)临床药学科,南京210002 [2]南方医科大学药学院,南京210002

出　　处：《分析化学》2021年第12期1945-1954,共10页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(No.61871403);江苏省杰出青年基金项目(No.BK20180005);江苏省青年医学重点人才项目(Nos.QNRC2016889,QNRC2016845)资助。

摘　　要：由病原体引起的传染性疾病严重威胁人类的生命与健康,快速、准确、即时地检测病原体是有效防控传染病的重要前提。核酸检测具有灵敏度高、特异性好等优点,成为病原体检测的主要技术。然而,传统的病原体核酸检测通常需要专业的技术人员和专门的仪器设备,不利于病原体的现场检测。利用纸基材料构建的生物传感器具有轻巧便携、成本低、结果直观等优点,与核酸扩增检测方法相结合,适用于病原体的现场快速检测。目前,纸基病原体核酸检测技术主要包括侧流层析试纸条和纸基微流控芯片两种类型。本文对侧流层析试纸条、纸基微流控芯片及其制备技术进行了评述,并对集病原体核酸提取、扩增与检测一体化的纸基装置进行介绍,以期为开发纸基病原体现场检测新技术提供参考。Infectious diseases caused by pathogens seriously threaten human life and health.Rapid and accurate detection of pathogens is an important prerequisite for effective prevention and control of infectious diseases.However,traditional methods for detection of pathogen nucleic acid often require professional personnel and specialized instruments and equipments,which challenges the realization of field detection of pathogen.Using paper as matrix to construct biosensor has the advantages of light and portable,low cost and intuitive results,and is suitable for rapid detection of pathogens in the field by combining with nucleic acid amplification method.Currently,the paper-based pathogen nucleic acid detection technique mainly includes two types:lateral flow strips and paper-based microfluidic devices.Here,antibody-dependent and antibody-independent lateral flow strips,two-and three-dimensional paper-based microfluidic devices and fabrication techniques are reviewed,and the strategies of developing paper-based integrated devices for nucleic acid extraction,amplification and detection of pathogens are introduced,which can provide a reference for the development of new techniques for the field detection of pathogens.

关 键 词：侧流层析试纸条 纸基微流控芯片 纸基一体化装置 病原体 核酸检测 评述 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置] R440[自动化与计算机技术—控制科学与工程]