多功能海藻酸钠海绵的制备及抗菌、止血性能分析  

Preparation and Analysis of Antibacterial and Hemostatic Properties of Multifunctional Sodium Alginate Sponge

作　　者：刘琳 班雨[1] 魏悦 王明哲 车超越[1] 石恒冲 张旭[1] 栾世方 LIU Lin;BAN Yu;WEI Yue;WANG Ming-Zhe;CHE Chao-Yue;SHI Heng-Chong;ZHANG Xu;LUAN Shi-Fang(State Key Laboratory of Polymer Physics and Chemistry,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China;University of Science and Technology of China,Hefei 230026,China;Shandong Weigao Orthopaedic Device Co.,Ltd.,Weihai 264200,China)

机构地区：[1]中国科学院长春应用化学研究所高分子物理与化学国家重点实验室,长春130022 [2]中国科学技术大学,合肥230026 [3]山东威高骨科材料股份有限公司,威海264200

出　　处：《分析化学》2021年第12期1986-1994,共9页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(No.51973221);山东省重大科技创新工程项目(No.2019JZZY011105);工信部生物医用材料生产示范平台项目(No.TC190H3ZV/1);中国科学院威高计划项目资助。

摘　　要：为避免不可控出血造成的失血性休克以及细菌滋生造成的伤口感染和菌血症,本研究以海藻酸钠(SA)与负载有环丙沙星(CIP)的埃洛石(HNTs)为原料,通过冷冻干燥制备了具有快速止血、高效抗菌性能的多功能海绵(CIP-HNTs/SAs)。利用红外(FTIR)光谱、扫描电子显微镜(SEM)、密度法和称重法对含有HNTs的SA海绵(HNTs/SAs)成分、多孔结构、孔隙率以及吸水率进行检测。利用凝血时间(BCT)和凝血指数(BCI)对HNTs/SAs止血性能进行检测并筛选出最优HNTs负载比例,同时通过活化部分凝血酶时间(APTT)和凝血酶原时间(PT)深入探究了海绵止血机制。利用能谱仪(EDS)以及FTIR光谱对负载CIP的HNTs(CIP-HNTs)进行成分表征并通过紫外(UV)吸收光谱对CIP-HNTs进行了CIP负载量的定量检测以及缓释性能检测。利用琼脂板计数法以及抑菌圈法对CIP-HNTs/SAs的长效抗菌性能进行检测,并利用细胞增殖检测试剂盒(CCK-8)以及红细胞溶血率对CIP-HNTs/SAs进行生物相容性检测。结果表明,此多功能海绵利用多孔结构、易吸水的特性以及海绵含有的Ca^(2+)和埃洛石协同作用,实现了体外18 s内快速凝血,同时可负载抗菌药物,并具有7天长效抗菌的优异性能。A multifunctional hemostatic sponge(CIP-HNTs/SAs)with rapid hemostasis and high efficiency antibacterial property was built by freeze-drying based on ciprofloxacin(CIP),halloysite(HNTs)and sodium alginate(SA).Fourier transform infrared(FTIR)spectroscopy,scanning electron microscope(SEM),density method and weighing method were used to detect the composition,porous structure,porosity and water absorption of the sodium alginate sponge(HNTs/SAs).Blood clotting time(BCT)and blood clotting index(BCI)were applied to test the hemostatic of HNTs/SAs and the optimal halloysite loading ratio was selected by these tests.Furthermore,the activated partial thrombin time(APTT)and prothrombin time(PT)were used to explore the mechanism of sponge hemostasis.The halloysite hollow tube structure could load with antibacterial drugs.The components of halloysite loaded with antibacterial drugs were characterized by energy dispersive spectroscopy(EDS)and FTIR spectroscopy,and the loaded antibacterial drugs were quantitatively detected by ultraviolet(UV)absorption spectrum.The long-term antibacterial activity of CIP-HNTs/SAs was tested by the agar plate counting methodand inhibition zone,and the biocompatibility of CIP-HNTs/SAs was tested by the cell proliferation test kit(CCK-8)and the hemolysis rate of red blood cells.The results showed that the porous and hydrophilia of sodium alginate sponge could quickly absorb water in the blood to promote blood cell aggregation.Moreover,calcium ions(Ca^(2+))and halloysite could enhance the body′s own coagulation cascade activity.Comparing with the coagulation time in vitro of the commercial gelatin hemostatic sponge,the sodium alginate sponge(HNTs/SA)containing halloysite could achieve rapid hemostasis in only 18 s.Meanwhile,CIP-HNTs/SAs had great antibacterial property and 7-day long-lasting antibacterial performance.

关 键 词：海藻酸钠 埃洛石 抗菌 快速止血 多功能海绵 

分 类 号：R318.08[医药卫生—生物医学工程]