新型纳米酶马达的制备及其抗菌性能研究  

Preparation of Novel Nanozyme-motor and Its Antibacterial Properties

作　　者：王唯 于滨 吕凯旋 孙文博 王大鹏[2,3] 逯乐慧[1,2] WANG Wei;YU Bin;LYU Kai-Xuan;SUN Wen-Bo;WANG Da-Peng;LU Le-Hui(State Key Laboratory of Electroanalytical Chemistry,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China;University of Science and Technology of China,Hefei 230026,China;State Key Laboratory of Polymer Physics and Chemistry,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China)

机构地区：[1]中国科学院长春应用化学研究所电分析化学国家重点实验室,长春130022 [2]中国科学技术大学应用化学与工程学院,合肥230026 [3]中国科学院长春应用化学研究所高分子物理与化学国家重点实验室,长春130022

出　　处：《分析化学》2021年第12期1995-2004,I0001-I0004,共14页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(No.21635007)资助。

摘　　要：利用聚多巴胺(PDA)和铜的螯合作用,设计合成了具有类过氧化氢酶和过氧化物酶活性的纳米酶马达(Cu/PDA)。Cu/PDA能够高效地催化过氧化氢分解产生氧气用于推动纳米马达运动,同时生成活性氧(ROS)自由基用于杀伤细菌。在多巴胺基质的光热效应促进下,纳米马达的扩散范围明显扩大,有效地增强了ROS的作用范围。多项抗菌实验结果表明,Cu/PDA可以高效地抑制细菌生长;并且,在表面进行特异性修饰后,Cu/PDA抗菌体系对耐药革兰氏阳性菌也表现出显著的抗菌活性。本研究通过马达运动的方式优化了活性氧类抗菌纳米酶的作用范围,解决了传统纳米酶杀菌效率受限的问题,对设计研发新型纳米抗菌剂具有参考价值。A nanozyme-motor(Cu/PDA)with peroxidase-like and catalase-like characteristics was constructed by in situ chelation between Cu^(2+) and polydopamine(PDA).Cu/PDA can efficiently catalyze the decomposition of hydrogen peroxide to produce oxygen for the movement of nano-motors and to generate highly toxic reactive oxygen species(ROS)for killing bacteria.Promoted by the photothermal effect of PDA,the diffusion range of nanozyme-motor was significantly expanded,which enhanced the damage range of ROS effectively.Antimicrobial experiments results showed that Cu/PDA could inhibit bacterial growth potently.Moreover,with the functional surface modification,Cu/PDA antibacterial system exhibited more distinct antimicrobial activity against the resistant Gram-positive bacteria.The damage range of ROS generated by antibacterial nanozyme was optimized through the combination with nanomotor,which addressed the issue on limited bactericidal efficiency of traditional nanozymes,and showed great potential in the development of new generation of antibacterial nanoagents.

关 键 词：纳米酶 纳米马达 活性氧 抗菌活性 

分 类 号：O629.8[理学—有机化学] TB383.1[理学—化学]