基于纳米金修饰硼化钴纳米片的电化学免疫传感器用于检测人绒毛膜促性腺激素  

An Electrochemical Immunosensor Based on Gold Nanoparticles Decorated Cobalt Boride Nanosheet Arrays for Detection of Human Chorionic Gonadotropin

作　　者：卢文卜 鲍灿灿 魏明 牛强强 白丽伟 曹小卫 贾建峰[1] LU Wen-Bo;BAO Can-Can;WEI Ming;NIU Qiang-Qiang;BAI Li-Wei;CAO Xiao-Wei;JIA Jian-Feng(Key Laboratory of Magnetic Molecules and Magnetic Information,Materials Ministry of Education,School of Chemistry and Material Science,Shanxi Normal University,Taiyuan 030035,China;Institute of Translational Medicine,Medical College,Yangzhou University,Yangzhou 225009,China)

机构地区：[1]山西师范大学化学与材料科学学院,磁性分子与磁信息材料教育部重点实验室,太原030035 [2]扬州大学医学院,转化医学研究中心,扬州225009

出　　处：《分析化学》2021年第12期2075-2085,I0019-I0023,共16页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(No.21705103);山西省应用基础研究计划项目(No.201801D221392);山西省高等学校科技创新项目(No.2019L0460);山西省1331工程重点学科建设计划项目资助。

摘　　要：开发了一种基于金纳米颗粒修饰硼化钴纳米片(AuNPs/CoB)阵列的电化学免疫传感器用于定量检测血清中的人绒毛膜促性腺激素(hCG)。通过电沉积与电聚合的方法构建硫堇/金纳米颗粒/硼化钴纳米片阵列,hCG抗体通过Au—N键或肽键偶联到硫堇/金纳米颗粒/硼化钴纳米片(Thi/AuNPs/CoB)阵列上形成免疫探针。金纳米颗粒修饰的硼化钴纳米片催化还原硫堇产生响应电流,实现对hCG的检测。构建的电化学免疫传感器具有高选择性和良好的稳定性,线性检测范围为10~5.0×10^(4) pg/mL,检出限(S/N=3)为2.8 pg/mL。实验结果表明,此电化学免疫传感器可应用于血清中hCG的检测,为早期妊娠和妇科疾病的临床诊断提供了一种简单可靠的评价方法。Human chorionic gonadotropin(hCG)is a marker of early pregnancy and diseases such as ovarian cancer.Therefore,the rapid,accurate and real-time determination of hCG is of great significance.In this work,an electrochemical immunosensor for detection of hCG based on three-dimensional Au nanoparticles anchored on cobalt boride(AuNPs/CoB)nanosheet array electrode was developed.AuNPs/CoB nanosheet arrays were synthesized by an electrodeposition method,which was first explored for immunoassay of hCG.AuNPs/CoB nanosheeet arrays could enhance the performance of electrocatalytic reduction of thionine to amplify signal.Thionine(Thi)was electro-polymerized on the AuNPs/CoB nanosheet arrays,thus forming Thi/AuNPs/CoB nanosheet array-electrode.HCG antibody(anti-hCG)could be successfully attached to the Thi/AuNPs/Co-B nanosheet array-electrode by Au—N bond of AuNPs or the amino group of thionine,which caused an obvious decrease in the catalytic current due to the insulated anti-hCG.The current signal decreased linearly with the increase of hCG concentration due to the formation of antibody-antigen immunocomplex.The immunosensor possessed a good linearity with logarithm of hCG concentration in the range of 10-5.0×10^(4) pg/mL,and the detection limit was 2.80 pg/mL(S/N=3).The immunosensor based on Thi/AuNPs/Co-B nanosheet arrays could exactly detect hCG in human serum samples,providing a simple and reliable evaluation method for the clinical diagnosis of early pregnancy and gynecological diseases.

关 键 词：硼化钴纳米片 阵列 金纳米颗粒 人绒毛膜促性腺激素 电化学免疫传感器 

分 类 号：R446.6[医药卫生—诊断学] O657.1[医药卫生—临床医学] TB383.1[理学—分析化学]