铂纳米粒子/碳纳米球基电化学传感器检测细胞释放的过氧亚硝基阴离子  

A Novel Electrochemical Sensor Based on Pt Nanoparticles/Carbon Nanospheres for Direct Detection of Peroxynitrite Anion Released by Living Cells

作　　者：刘福鑫 杜世龙 李琳 刘倩 丁兰 刘秀辉 LIU Fu-Xin;DU Shi-Long;LI Lin;LIU Qian;DING Lan;LIU Xiu-Hui(Key Laboratory of Bioelectrochemistry&Environmental Analysis of Gansu Province,College of Chemistry&Chemical Engineering,Northwest Normal University,Lanzhou 730070,China)

机构地区：[1]西北师范大学化学化工学院,甘肃省生物电化学与环境分析重点实验室,兰州730070

出　　处：《分析化学》2021年第12期2086-2095,I0024-I0029,共16页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(No.21565021)资助。

摘　　要：过氧亚硝基阴离子(ONOO-)是生物系统中一种重要的分子,在正常生理条件下,其在体内的浓度较低。然而,氧化应激可使机体产生高浓度的ONOO-,严重破坏生物分子,影响细胞的生理功能。因此,构建可灵敏快速检测ONOO-的传感器非常重要。本研究基于铂纳米粒子(PtNPs)修饰的碳纳米球(CNS)复合材料,构建了一种新型的ONOO-电化学传感器。采用扫描电子显微镜和透射电子显微镜对材料的形貌进行了表征,通过循环伏安法研究了CTS/PtNPs/CNS/GCE传感器的电化学性能。在最佳实验条件下,此传感器对ONOO-的检测表现出优异的分析性能,具有宽的线性范围(0.615 nmol/L~0.139 mmol/L)和超低的检出限(0.205 nmol/L(S/N=3)),可用于检测细胞释放的ONOO-。进一步探究了Cd^(2+)浓度对诱导细胞释放ONOO-的影响,研究了在Cd^(2+)诱导下α-硫辛酸(ALA)和还原型谷胱甘肽(GSH)对细胞氧化损伤的保护作用,发现混合抗氧化剂(ALA+GSH)能更好地保护细胞。此抗氧化策略有望用于生物医学领域和癌症疾病的治疗。Peroxynitrite anion(ONOO-)is one of the most important molecules in biological systems and is usually maintained at a very low concentration level under normal physiological condition.However,under oxidative stress,the body will produce high concentration of ONOO-abnormally,which may damage many biomolecules seriously and affect the physiological functions of cells.Therefore,it is very important to construct a sensor that can detect ONOO-released by cells sensitively and rapidly.In this work,a novel ONOO-electrochemical sensor was constructed based on carbon nanospheres(CNS)composite materials modified with platinum nanoparticles(PtNPs).Electron microscopes and electrochemical technology were used for characterization of the prepared CTS/PtNPs/CNS.The sensor showed excellent analytical performance for ONOO-detection,with a wide linear range from 0.615 nmol/L to 0.139 mmol/L,and an extremely low detection limit of 0.205 nmol/L(S/N=3).In addition,it also showed good selectivity,stability and reproducibility,and was applied to detect ONOO-released from living cells.The released ONOO-of cells induced by Cd^(2+)was investigated,and it was found that more ONOO-released from cells at higher Cd^(2+)concentration.Finally,the protective effects of theα-lipoic acid(ALA)and glutathione(GSH)on cells were investigated by electrochemical method,and it was found that the antioxidants mixture(ALA+GSH)had better protect cells due to their synergistic effect.The experimental results showed that the developed sensing platform and efficient antioxidation strategy provided the possibility for the future application in the biomedical field and cancer diseases.

关 键 词：过氧亚硝基阴离子 电化学传感器 碳纳米球 抗氧化剂 铂纳米粒子 活细胞 

分 类 号：R446[医药卫生—诊断学] O657.1[医药卫生—临床医学] TB383.1[理学—分析化学]