醇酸体系分离木质素对酶解性能的影响  

Effect of lignin separation by alcohol and acid system on enzymatic hydrolysis performance

作　　者：安胜欣[1] 薛凤洋 李文志 AN Sheng-xin;XUE Feng-yang;LI Wen-zhi(School of Chemical Engineering,Anhui University of Science and Technology,Huainan 232001,Anhui Province,China;Department of Thermal Science and Energy Engineering,University of Science and Technology of China,Hefei 230026,Anhui Province,China)

机构地区：[1]安徽理工大学化学工程学院,安徽淮南232001 [2]中国科学技术大学工程科学学院,安徽合肥230026

出　　处：《化学工程》2021年第9期3-8,共6页Chemical Engineering(China)

基　　金：安徽省重点研发项目(202004a06020053);国家重点基础研究发展计划(2018YFB1501601)。

摘　　要：为提高生物质纤维素酶解性能,采用乙二醇耦合对甲基苯磺酸(EG-PTSA)法对玉米秸秆进行预处理,考察了温度、对甲基苯磺酸(PTSA)质量分数、反应时间对酶解性能的影响,并通过扫描电镜(SEM)、X射线衍射(XRD)和傅里叶变换红外光谱仪(FTIR)对预处理前后的样品进行表征分析。结果表明:在对甲基苯磺酸质量分数2.0%、100℃、60 min下,木质素脱除率为83.2%,纤维素保留率为83.5%;同时,酶解纤维素的葡聚糖产率从23.8%提高至71.4%(5 FPU/g),比原始玉米秸秆的酶解效率(23.8%)高出近3倍。SEM、XRD和FTIR分析表明,玉米秸秆经EG-PTSA预处理后,木质素、半纤维素大量脱除打破了秸秆纤维素致密结构,增加了纤维素酶的可及性,提高了酶解性能,是一种简单、高效且有前景的预处理方法。In order to improve the enzymatic hydrolysis performance of biomass cellulose,ethylene glycol coupled with p-toluenesulfonic acid(EG-PTSA)was adopted to pretreat corn stover.The effects of temperatures,PTSA mass fraction and reaction time on the enzymatic hydrolysis performance of corn stover were investigated.The structures of the samples before and after pretreatment were characterized by scanning electorn microscopy(SEM),X-ray diffraction(XRD)and fourier transform infrared spectroscopy(FTIR).The results show that 83.2%delignification and 83.5%retention of cellulose are obtained under the selected conditions of p-toluenesulfonic acid mass fraction 2.0%,100℃and 60 min.Meanwhile,the enzymatic hydrolysis sugar yield significantly increases to 71.4%(5 FPU/g),which is nearly 3 times higher than that of untreated corn stover(23.8%).The analysis of SEM,XRD and FTIR shows that the dense structure of corn stover cellulose is seriously destroyed because of the massive removal of lignin and hemicellulose,which markedly improves cellulase accessibility to cellulose and lead to the high efficiency of enzymatic hydrolysis.The present study shows that the pretreatment is a simple,efficient and promising process for corn stover biorefinery.

关 键 词：生物质 预处理 乙二醇 对甲基苯磺酸 木质素 

分 类 号：TQ021.1[化学工程] TQ021.3