重庆加工型辣椒种质资源抗疫病鉴定的分子标记筛选  

Molecular Marker Screening of Processed Pepper (Capsicum annuum) Germplasm Resources against with Phytophthora capsici in Chongqing

作　　者：王春萍[1] 杨小苗 李怡斐[2] 雷开荣[1] 黄启中[2] 黄任中[2] 林清[1] 吴红[1] 张世才[2] WANG Chunping;YANG Xiaomiao;LI Yifei;LEI Kairong;HUANG Qizhong;HUANG Renzhong;LIN Qing;WU Hong;ZHANG Shicai(Chongqing Key Laboratory of Adversity Agriculture,Biotechnology Research Institute,Chongqing Academy of Agricultural Sciences,Chongqing 401329,China;Vegetable and Flower Research Institute,Chongqing Academy of Agricultural Sciences,Chongqing 401329,China)

机构地区：[1]重庆市农业科学院生物技术研究所逆境农业研究重庆市市级重点实验室,重庆401329 [2]重庆市农业科学院蔬菜花卉研究所,重庆401329

出　　处：《热带亚热带植物学报》2021年第6期642-648,共7页Journal of Tropical and Subtropical Botany

基　　金：国家现代农业产业技术体系建设专项资金项目(CARS-24-A-04);重庆市技术创新与应用发展专项项目(cstc2020jscx-msxmX0054;cstc2019jscx-gksbX0123)资助。

摘　　要：为筛选出适用于重庆加工型辣椒疫病抗性鉴定的分子标记,以63份重庆加工型辣椒种质资源为材料,研究了12个辣椒疫病抗性相关分子标记的筛选效率。结果表明,7个分子标记在种质间无差异条带,2个分子标记的扩增结果不稳定,只有ZL6203、ZL6726和E73等3个分子标记在种质间能扩增出差异条带。ZL6203筛选高抗、抗性和中抗材料的效率分别为87.50%、77.78%和63.64%;ZL6726筛选抗性和感病材料的效率分别为100.00%和66.67%;E73筛选抗性和高抗材料的效率分别为77.78%和62.50%。因此,同一辣椒抗疫病分子标记对不同抗性材料筛选效率存在较大差异,ZL6203、ZL6726和E73适用于重庆地区加工型辣椒抗疫病材料的鉴定。To screen out molecular markers suitable for identification of Phytophthora capsici resistant pepper in Chongqing, the screening efficiency of 12 molecular makers for Phytophthora blight resistance was studied in 63 processed pepper(Capsicum annuum) germplasms in Chongqing. The results showed that there were no different amplification bands of 7 markers among the pepper germplasms, and the amplification results of 2 markers were unstable. Only 3 markers, such as ZL6203, ZL6726 and E73, could amplify different bands among pepper germplasms. For ZL6203, the efficiency of screening high resistant, resistant and medium resistant materials was 87.50%, 77.78% and 63.64%, respectively. For ZL6726, the efficiency of screening resistant and susceptible materials was 100.00% and 66.67%, respectively, and for E73, the efficiency of screening resistant and high resistant materials was 77.78% and 62.50%, respectively. Therefore, the screening efficiency of the same molecular marker for different resistance materials was quite different. ZL6203, ZL6726 and E73 markers were suitable for identification of Phytophthora blight resistant of processed pepper in Chongqing.

关 键 词：辣椒 种质资源 疫病 分子标记 

分 类 号：S436.418.1[农业科学—农业昆虫与害虫防治]