基于网络药理学探讨星蒌承气汤治疗急性脑梗死的作用机制  

Exploration on the Action Mechanism of Xinglou Chengqi Decoction in the Treatment of Acute Cerebral Infarction Based on Network Pharmacology

作　　者：陈琛 李伟[1] 耿花蕾 王雅惠 吴康 王月 陈天竹 邹忆怀[1] CHEN Chen;LI Wei;GENG Hualei;WANG Yahui;WU Kang;WANG Yue;CHEN Tianzhu;ZOU Yihuai(Dongzhimen Hospital of Beijing University of Chinese Medicine,Beijing 100700,China)

机构地区：[1]北京中医药大学东直门医院,北京100700

出　　处：《世界中医药》2021年第21期3161-3167,共7页World Chinese Medicine

基　　金：国家自然科学基金面上项目(81873257);国家“十二五”科技支撑计划项目(2013BAI13B022)。

摘　　要：目的:基于网络药理学探讨星蒌承气汤治疗急性脑梗死的作用机制。方法:通过中药系统药理学数据库与分析平台(TCMSP)结合文献检索获取星蒌承气汤有效药物成分;在Swiss Target Prediction数据库预测药物成分相关靶点;利用在线人类孟德尔遗传(OMIM)数据库、GeneCards数据库获取急性脑梗死疾病相关靶点;绘制韦恩图得到星蒌承气汤治疗急性脑梗死的潜在作用靶点;采用String数据库、Cytoscape 3.8.0软件构建潜在作用靶点蛋白质-蛋白质相互作用网络、药物-成分-疾病-靶点网络;使用DAVID 6.8数据库对潜在作用靶点进行基因本体(GO)及京都基因与基因组百科全书(KEGG)富集分析。结果:通过TCMSP结合相关文献检索共获得星蒌承气汤药物有效成分47个,包括芹菜素、香叶木素、胆酸等;得到星蒌承气汤治疗急性脑梗死潜在作用靶点85个,包括STAT3、ESR1、CCNB1等;共获得GO富集分析功能条目225条,包括生物过程(BP)132条,细胞组分(CC)34条,分子功能(MF)59条;KEGG信号通路共20条,主要包括花生四烯酸代谢、胆汁分泌、cAMP信号通路等。结论:基于网络药理学,从分子层面阐释了星蒌承气汤治疗急性脑梗死的机制可能为抑制氧化应激、抗炎、调节能量代谢、抗凋亡等,为后续实验研究、新药研发提供了线索和依据。Objective:To explore the mechanism of Xinglou Chengqi Decoction in the treatment of acute cerebral infarction based on network pharmacology.Methods:Pharmacology database and analysis platform of traditional Chinese medicine system platform(TCMSP)combined with literature retrieval was used to obtain the effective drug components of Xinglou Chengqi Decoction;SwissTargetPrediction database was used to predict drug components related targets;OMIM and GeneCards database were used to obtain the disease-related targets of acute cerebral infarction;we drew a Venn diagram to get the potential target of Xinglou Chengqi Decoction in the treatment of acute cerebral infarction.STRING database and Cytoscape 3.8.0 software were used to construct potential target protein interaction network and drug-component-disease-target network;GO and KEGG enrichment analysis of potential targets were carried out using David 6.8 database.Results:Through TCMSP and related literature search,47 effective components of Xinglou Chengqi Decoction were obtained,including apigenin,vanillin,cholic acid,etc.A total of 85 potential targets of Xinglou Chengqi Decoction in treating acute cerebral infarction were obtained,including STAT3,ESR1,CCNB1,etc.A total of 225 functional items of GO enrichment analysis were obtained,including 132 biological process(BP),34 cell components(CC)and 59 molecular function(MF).There were 20 KEGG signaling pathways,including arachidonic acid metabolism,bile secretion,cAMP signaling pathway etc.Conclusion:Based on network pharmacology,the mechanism of treatment of acute cerebral infarction by Xinglou Chengqi Decoction is explained from the molecular level,which may be used to inhibit oxidative stress,anti-inflammatory,regulate energy metabolism,anti-apoptosis,etc,and provide clues and basis for subsequent experimental research and development of new drugs.

关 键 词：急性脑梗死 星蒌承气汤 网络药理学 作用机制 

分 类 号：R285[医药卫生—中药学]