氧化应激对慢性阻塞性肺疾病上皮-间质转化的影响  

The Effect of Oxidative Stress on Epithelial-mesenchymal Transition in Chronic Obstructive Pulmonary Disease

作　　者：胡进秀 崔节达 郭晓桐[3] 郝斌威 陈娟[3] HU Jinxiu;CUI Jieda;GUO Xiaotong;HAO Binwei;CHEN Juan(School of Clinical Medicine,Ningxia Medical University,Yinchuan 750004,China;Department of Respiratory and Critical Care Medicine,Zhongwei People's Hospital,Zhongwei 755000,China;Department of Respiratory and Critical Care Medicine,General Hospital of Ningxia Medical University,Yinchuan 750004,China;Department of Respiratory and Critical Care Medicine,Shanxi Bethune Hospital,Shanxi Academy of Medical Sciences,Taiyuan 030000,China)

机构地区：[1]宁夏医科大学临床医学院,银川750004 [2]宁夏中卫市人民医院呼吸与危重症医学科,中卫755000 [3]宁夏医科大学总医院呼吸与危重症学科,银川750004 [4]山西白求恩医院,山西医学科学院呼吸与危重症医学科,太原030000

出　　处：《宁夏医科大学学报》2021年第11期1135-1140,共6页Journal of Ningxia Medical University

基　　金：国家自然科学基金项目(81760004);呼吸疾病国家重点实验室2019年度开放课题(SKLRD-OP-201903);宁夏自然科学基金重点项目(2020AAC02001);第四批宁夏青年科技人才托举工程(TJGC2019101);宁夏医科大学校级科研项目(XM2020149);第四批宁夏青年科技人才托举工程(TJGC2019101);宁夏医科大学校级科研项目(XM2020149)。

摘　　要：目的探究氧化应激与慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)上皮-间质转化(epithelial-mesenchymal transition,EMT)的关系。方法收集2015年1月至2017年12月宁夏医科大学总医院心胸外科因肺部肿瘤需行肺叶切除的患者,按2017 GOLD指南分为肺功能正常(对照)组(20例)和COPD组(16例)。通过HE染色观察COPD组及对照组小气道形态学的改变;利用免疫组织化学方法分别检测COPD组及对照组肺组织中EMT标记物E-钙粘连蛋白(E-cadherin,E-Ca)及纤维连接蛋白(fibronectin,FN)的表达情况;采用蛋白免疫印迹法检测COPD组及对照组肺组织中EMT标记物E-Ca和FN的表达情况;采用酶联免疫吸附剂测定法检测COPD组及对照组血清中8-异前列腺素F2α(8-iso-PGF2α)和总抗氧化能力(T-AOC)的浓度。结果免疫组织化学染色结果显示,COPD组气道上皮存在明显的EMT现象,COPD组间质表型标记物FN表达(0.156±0.043)高于对照组(0.072±0.058)(P=0.035),COPD组上皮表型标记物E-Ca表达(0.142±0.068)低于对照组(0.256±0.067)(P=0.016);免疫印迹结果显示,COPD组FN表达(0.60±0.22)高于对照组(0.39±0.22)(P=0.026),COPD组E-Ca表达(0.36±0.20)低于对照组(0.84±0.46)(P=0.003);COPD组血清(8-iso-PGF2α)浓度(45.91±21.17)pg·mL-1高于对照组(29.74±15.88)pg·mL-1(P=0.013),而COPD组血清(T-AOC)浓度(8.49±3.43)IU·mL-1低于对照组(13.35±4.28)IU·mL-1(P=0.001)。结论COPD组织内存在氧化应激及明显的EMT现象,且氧化应激可能通过某种机制参与调控COPD的发生、发展。Objective To explore the association between oxidative stress and epithelial-mesenchymal transition(EMT)in chronic obstructive pulmonary disease(COPD).Methods Data was gathered from a group of patients undergoing lobectomy for lung tumors admitted to the Department of Cardiothoracic Surgery,General Hospital of Ningxia Medical University from January 2015 to December 2017.According to the GOLD guidelines,patients were divided into normal lung function group(abbreviated as control group,20 cases)and COPD group(abbreviated as COPD group,16 cases).HE staining was used to observe the airway morphology changes in the COPD group and the control group;Immunohistochemical methods were used to detect the expression of EMT markers E-cadherin(E-Ca)and fibronectin(FN)in the lung tissues of the COPD group and the control group,respectively;Western blotting was used to detect The expression of EMT markers E-Ca and FN in the lung tissues of the COPD group and the control group;The enzyme-linked immunosorbent assay was used to detect the expression of 8-isoprostaglandin F2α(8-iso-PGF2α)and total antioxidant capacity(T-AOC)in serum samples of both the COPD group and Control group.Results Immunohistochemical staining showed that the airway epithelium of the COPD group had obvious EMT phenomenon.The expression of mesenchymal phenotype marker FN in the COPD group(0.156±0.043)was higher than that of the control group(0.072±0.058)(P=0.035),and The expression of marker of the epithelial phenotype of the COPD group E-Ca(0.142±0.068)was lower than that of the control group(0.256±0.067)(P=0.016);Western blot also showed that the expression of FN in the COPD group(0.60±0.22)was higher than that of the control group(0.39±0.22)(P=0.026),and the expression of E-Ca in the COPD group(0.36±0.20)was lower than that of the control group(0.84±0.46)(P=0.003).The oxidation product 8-iso-prostaglandin F2α(8-iso-PGF2α)serum concentration of COPD group(45.91±21.17)pg·mL-1 was higher than that of the control group(29.74±15.88)pg·mL-1(P=0.0

关 键 词：慢性阻塞性肺疾病 氧化应激 上皮-间质转化 

分 类 号：R563[医药卫生—呼吸系统]