医用胶水应用换药对小鼠急性创面愈合速度的对比研究  

Comparative study on acute wound in mice by using medical glue for dressing change

作　　者：曹亿[1] 彭吉才[1] 钟广正 朱颉[1] CAO Yi;PENG Ji-cai;ZHONG Guang-zheng;ZHU Jie(Department of Emergency,Sun Yat-sen Memorial Hospital,Sun Yat-sen Uni-versity,Guangzhou 510288,China;Department of Urology,Sun Yat-sen Memorial Hospital,Sun Yat-sen Uni-versity,Guangzhou 510288,China)

机构地区：[1]中山大学孙逸仙纪念医院急诊科,广州510288 [2]中山大学孙逸仙纪念医院泌尿外科,广州510288

出　　处：《岭南现代临床外科》2021年第5期556-560,共5页Lingnan Modern Clinics in Surgery

基　　金：国家自然科学基金(81802552)。

摘　　要：目的探讨医用胶水的创面应用与传统换药方法在小鼠急性皮肤撕脱创面的修复愈合速度方面有无差异。方法18只C57小鼠随机分为三组制作急性皮肤撕脱创面,每组各6只,分别为医用胶组、凡士林组、对照组,2天换一次药,共10天。分别于第0、2、6、10天大体观察创面愈合情况;免疫印迹(Western blotting)及酶联免疫(ELISA)检测炎症介质IL⁃6、TNF⁃α表达情况。第10天免疫组化观察IL⁃6、TNF⁃α表达情况。结果第2、6、10天观察创面修复情况,医用胶组修复最快,其次为对照组,凡士林组修复最慢;免疫印迹及酶联免疫检测IL⁃6、TNF⁃α的表达情况,医用胶组表达最少,其次为对照组,凡士林组表达最多。第10天免疫组化观察IL⁃6、TNF⁃α表达量,医用胶组表达最少,其次为对照组,凡士林组表达最多。结论与传统换药方法相比,医用胶水具有减少炎性介质、加快急性皮肤撕脱创面修复愈合的作用。Objective To investigate whether the healing speed of acute skin avulsion wound in mice was different between medical glue and traditional dressing change.Methods Eighteen C57 mice were randomly divided into three groups to make acute skin avulsionwounds,six mice in each group,namely medical glue group,vaseline group,and control group.The dressing were changed every 2 days for a total of 10 days.On the 0 th,2 th,6 th and 10 th days,the condition of wound healing was observed,the expressions of inflammatory mediators IL⁃6 and TNF⁃αwere detected by Western blotting and ELISA respectively.On the 10 th day,the expression of IL⁃6 and TNF⁃αwere observed by immunohistochemis⁃try.Results On the 2 th,6 th and 10 th days,the healing speed of the glue group was the fastest,followed by the control group,and the vaseline group was the slowest.For the expressions quantity of the IL⁃6 and TNF⁃αby Westernblotting and ELISA,the glue group was the lowest,followed by the control group,and the vaseline group was the highest.On the 10 th day,for the expression levels of IL⁃6 and TNF⁃αbyimmunohistochemistry,the glue group was the lowest,followed by the control group,and the vaseline group was the highest.Conclusion Compared with traditional dressing change,medical glue can reduce inflammatory mediatorsand accelerate the healing of acute skin avulsion wound.

关 键 词：医用胶 IL⁃6 TNF⁃α 急性创面 

分 类 号：R641[医药卫生—外科学]